现代医药卫生2023，Vol.39Issue(23)：3977-3981,5.DOI:10.3969/j.issn.1009-5519.2023.23.004
PMQR与耐碳青霉烯类肠杆菌科细菌喹诺酮耐药机制的关系
The relationship between PMQR and mechanism of carbapenem-resistant enterobacteriaceae to quinolones
冯霞 1刘小云 1许晶晶2
作者信息
- 1. 徐州医科大学附属医院中心实验室,江苏徐州 221002
- 2. 徐州医科大学附属医院检验科,江苏徐州 221002
- 折叠
摘要
Abstract
Objective To analyze the distribution of plasmid-mediated quinolone resistance gene(PMQR)in carbapenem-resistant enterobacteriaceae(CRE),and to explore the mechanism of quinolone resist-ance.Methods A total of 43 non-repetitive CRE strains isolated from the hospital from July 2021 to Septem-ber 2022 were collected.The automatic microbial analysis system was used for strain identification and drug sensitivity test.The PMQR,carbapenemase and β-lactamase resistance genes were detected by polymerase chain reaction(PCR),and the quinolone resistance mechanism of CRE strains was analyzed.Results The drug resistant rates of 31 strains of carbapenem-resistant escherichia coli to quinolones were 93.55％-96.77％.The drug resistant rates of 12 strains of carbapenem-resistant Klebsiella pneumoniae to quinolones were 66.67％-83.33％.The drug resistant rates of 43 CRE strains to β-lactamase blockers were 79.07％-100.00％.It was 100.00％sensitive to polycolistin and tigacycline.The drug resistant rates of amikacin and tobramycin to polymyxin and tigecycline and aminoglycosides were low.Among the 43 CRE strains,88.37％carried one or two PMQRs,of which the detection rate of acc(6')Ib-cr was 83.72％,the detection rate of qnrS was 69.77％,the detection rate of qnrB was 2.33％,and qnrA and qepA were not detected.Carbapenemase resistance genes were mainly NDM(88.97％)and KPC(32.56％).β-lactamase resistance genes were mainly SHV(83.23％)and CTX-M15(67.57％).Conclusion The detection rate of PMQR in CRE strains is high,which is related to the quinolone resistance mechanism of CRE,and may lead to multidrug resistance with the combined action of NDM,KPC and CTX-M15.关键词
耐碳青霉烯类肠杆菌科细菌/质粒介导/喹诺酮耐药基因/耐药机制Key words
Carbapenem-resistant enterobacteriaceae/Plasmid-mediated/Quinolone resistance gene/Mechanism分类
医药卫生引用本文复制引用
冯霞,刘小云,许晶晶..PMQR与耐碳青霉烯类肠杆菌科细菌喹诺酮耐药机制的关系[J].现代医药卫生,2023,39(23):3977-3981,5.
