西南林业大学学报2023，Vol.43Issue(11)：135-140,6.DOI:10.11929/j.swfu.202209002
松材线虫侵染对日本落叶松生理指标的影响
Effects of Bursaphelenchus xylophilus Infestation on Physiological Indexes of Larix kaempferi
摘要
Abstract
The dynamic changes of photosynthetic pigment content,osmotic regulatory substances,phenylalanine ammonia lyase and antioxidant enzyme system were determined to explore the response of Larix kaempferi to the infection of Bursaphelenchus xylophilus from the physiological and biochemical perspectives.Three-year-old potted L.kaempferi was used as the experimental material.Two thousand B.xylophilus were in-oculated by artificial skin grafting method.The chlorophyll content,soluble protein content,antioxidant enzymes(superoxide dismutase SOD,catalase CAT and peroxidase POD)activities and phenylalanine ammonia lyase(PAL)activities were collected and measured on the 0,5,10,20,and 30 days after inoculation.The results showed that a large number of B.xylophilus were detected in the upper and lower parts of the inoculated site of L.kaempferi,and the number of nematodes in the lower part was significantly higher than that in the upper part(P＜0.01).The contents of chlorophyll a and b both decreased significantly on the 10th day,and then began to increase,which had no significant difference with the control group.After 10 days of inoculation,the content of soluble protein gradually increased and was significantly higher than that of the control group(P＜0.05).The SOD activity increased significantly after B.xylophilus inoculation,which was significantly higher than that of the con-trol group at each time point(P＜0.01).CAT activity decreased slightly at first and then increased gradually,which was significantly higher than that in the control group at 30 days(P＜0.01).POD activity decreased first and then increased,and increased rapidly after 5 days,which was significantly higher than that of the control group at each time point(P＜0.05).The activity of PAL increased at first and then decreased.The activity at 10 and 20 days after inoculation was significantly higher than that in the control group(P＜0.05).The changes of chlorophyll content,soluble protein content,antioxidant enzymes and PAL activities were closely related to the disease resistance of L.kaempferi infected with B.xylophilus.The results of this study can provide a reference for the screening of resist-ance lines and the resistance mechanism of L.kaempferi infected with B.xylophilus.关键词
松材线虫/日本落叶松/抗氧化酶/生理响应/苯丙氨酸解氨酶Key words
Bursaphelenchus xylophilus/Larix kaempferi/antioxidant enzyme/physiological response/phenylalanine ammonia lyase分类
农业科技引用本文复制引用
王建国,姜旭,栾庆书,冯健,王建军,刘穆..松材线虫侵染对日本落叶松生理指标的影响[J].西南林业大学学报,2023,43(11):135-140,6.基金项目
辽宁省自然科学基金项目(2021-MS-053)资助 （2021-MS-053）
辽宁省农业科学院院长基金(2020QN2420)资助 （2020QN2420）
国家自然科学基金项目(32071674,31971652)资助. （32071674,31971652）
