西南林业大学学报2023，Vol.43Issue(11)：192-198,7.DOI:10.11929/j.swfu.202207016
1株云南楚雄野生香菇菌株的分离鉴定及袋料栽培方法研究
Isolation and Identification of a Wild Lentinula edodes Strain in Chuxiong,Yunnan and Bag Cultivation
摘要
Abstract
In the research,a wild Lentinula edodes strain from Yunnan Chuxiong area was isolated and char-acterized.Effect of different temperature,pH,carbon source,nitrogen source and inorganic salt on mycelia growth of the strain were studied.At the same time,the wild L.edodes strain was successfully cultivated by the mixed sawdust,corn cob and other agricultural waste resources as the main substrate.The results were as follows:Com-bined with morphological and ITS molecular identification,the strain was identified as Lentinula fungus by mor-phological and molecular identification;biological characteristics experiments found that the optimum temperat-ure for the growth of wild L.edodes mycelium was 30.0 ℃,and the optimum pH was 6.0,the best carbon source and nitrogen source are corn meal and peptone,the best inorganic salt are calcium chloride and magnesium sulfate;bag substitute material cultivation results show:the optimal bag substitute composed of 60.0％sawdust and 30.0％corncob,the biotransformation rate reached 41.49％.关键词
野生香菇/菌种分离/菌种鉴定/生物学特性/袋料栽培Key words
Wild Lentinula edodes/strain isolation/strain identification/biological characteristics/bag sub-stitute material cultivation分类
农业科技引用本文复制引用
寸孟人,余浪,李蕾,王琴,陈玉惠,冯小飞..1株云南楚雄野生香菇菌株的分离鉴定及袋料栽培方法研究[J].西南林业大学学报,2023,43(11):192-198,7.基金项目
国家级大学生创新创业训练计划项目(202110677014)资助 （202110677014）
云南省大学生创新创业训练计划项目(202110677007,202010677019)资助 （202110677007,202010677019）
生物学质量工程项目(503190106)资助 （503190106）
云南省教育厅科学研究基金项目(2022J0506)资助. （2022J0506）
