刑事技术2023，Vol.48Issue(6)：602-609,8.DOI:10.16467/j.1008-3650.2023.0024
Ion Ampliseq HID Y-SNP Research Panel V1试剂盒的法医学验证
Forensic Validation of the Ion AmpliSeq HID Y-SNP Research Panel V1 Kit
蔡信 1王凯旋 1缪路 1朱久亮 1刁中杰 2吴静 2徐伟1
作者信息
- 1. 淮南市公安局刑侦支队,安徽淮南 232000
- 2. 安徽宏健瑞齐生物科技有限公司,合肥 230031
- 折叠
摘要
Abstract
Detecting of the single-nucleotide polymorphisms of Y-chromosome(Y-SNP)is particularly useful in the identification of paternal lineages for forensic evidences under investigation.To evaluate the technical performance in Y-SNP detection and its forensic applicability of the Ion AmpliSeq HID Y-SNP Research Panel V1 Kit,this study tested the kit from the aspects of sequencing performance,repeatability and sensitivity using pedigree samples and the Control DNA 9948.Forty four samples from one male family with clear genetic relationships but have Y-STR mutations were detected by Ion AmpliSeq HID Y-SNP Research Panel V1 Kit on the Ion GeneStudio S5 Plus next generation sequencing platform,and serials diluted 9948 DNA samples were also sequenced in parallel.The sequencing results showed that each run of sequencing produced no less than1.97 Gb of data,more than 99％of the sequences in alignment.An average of 845 Y-SNP(98.37％)loci could be used for subsequent analysis,and the sequencing results were proved to be reliable.The Y-SNP sequencing results of the Control DNA 9948 were consistent,repeatable,and the sensitivity of this Y-SNP detecting kit could be as low as 100 pg.High resolution Y-SNP haplogroups were obtained for all the pedigree samples tested,and the Y-SNP haplogroup analysis results were consistent.Therefore,the Ion Ampliseq HID Y-SNP Research Panel V1 Kit has excellent sequencing performance,high sensitivity and good repeatability,and can be used as a supplementary method for pedigree screening in forensic casework.关键词
法医遗传学/家系排查/单倍群/Y染色体单核苷酸多态性/二代测序Key words
forensic genetics/pedigree screening/haplogroup/Y-SNP/next generation sequencing分类
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蔡信,王凯旋,缪路,朱久亮,刁中杰,吴静,徐伟..Ion Ampliseq HID Y-SNP Research Panel V1试剂盒的法医学验证[J].刑事技术,2023,48(6):602-609,8.
