刑事技术2023，Vol.48Issue(6)：610-615,6.DOI:10.16467/j.1008-3650.2023.0018
QTRAPLC-MS/MS法检测血、尿中莫达非尼
Determination of Modafinil in Blood and Urine by QTRAP LC-MS/MS
摘要
Abstract
In this paper,a method for the determination of modafinil in blood and urine was established by ultra-performance liquid chromatography-tandem hybrid-quadrupole-linear ion trap mass spectrometry.Carbamazepine was selected as the internal standard,and blood and urine were extracted with methanol/acetonitrile(V/V,1∶1),then centrifuged at high speed,and filtrated by membrane.Using 0.1％(V/V)formic acid/water solution and 0.1％(VN)formic acid/acetonitrile as mobile phase,the mixture was separated on Phenomenex Kinetex C18(2.1 cm×100 mm×2.6 μm)column.Electrospray positive ion monitoring/information-dependent collection/enhanced ion scanning(MRM-IDA-EPI)and secondary library retrieval were used for analysis.In the range of 1-80 ng/mL,modafinil had a good linear relationship,and the linear correlation coefficient was greater than 0.997.The limit of detection(S/N≥3)of blood and urine was 0.5 ng/mL,and the lower limit of quantifation(S/N≥10)was 0.8 ng/mL.At 2,20,80 ng/mL,the extraction efficiency of the blood and urine were 80.1％-110.3％and 87.9％-109.1％respectively and the RSD was less than 10％.This method can be applied to the qualitative and quantitative analysis of modafinil in blood and urine in cases.关键词
法医毒物学/三重四极杆/线性离子阱质谱/多反应监测-信息依赖性采集-增强离子扫描/莫达非尼Key words
forensic toxicology/triple quadrupole/linear ion trap mass spectrometry/MRM-IDA-EPI/modafinil分类
政治法律引用本文复制引用
邸玉敏,肖楠,王瑞花,周欣欣,常靖,张景然..QTRAPLC-MS/MS法检测血、尿中莫达非尼[J].刑事技术,2023,48(6):610-615,6.基金项目
中央级公益性科研院所基本科研业务费专项资金项目(2021JB007) （2021JB007）
