刑事技术2023，Vol.48Issue(6)：616-622,7.DOI:10.16467/j.1008-3650.2023.0046
DNATyperTM 30六色荧光STR复合扩增冻干试剂的验证
Comparation of Lyophilized and Normal DNATyperTM 30 6-dye PCR Amplification Kit
摘要
Abstract
Freeze drying technology can remove about 99％moisture from the substance,resulting in the freeze-dried product with an increased internal surface area,which is loose,porous,and easy to recover.These characteristics are very suitable for application in the PCR amplification kit of forensic STR examination.Lyophilized reagent could break the challenge of the upper limit of template addition in conventional PCR systems.This study aims to test the technical performance of DNATyperTM 30 lyophilized reagent and evaluate its application ability in practical forensic cases.In order to test the detection rate,sensitivity,stability and other aspects of lyophilized reagents,an exploration was carried out by setting different storage conditions,time ranges and concentration gradient.In order to meet the needs of room temperature transportation,the reagents were placed at room temperature(23 ℃ to 25 ℃)to simulate the transportation environment and compare the STR typing results at 1,7,15,30 days.In sensitivity test,standard DNA 9947A was used as template and its gradient was set from 0.0313 ng to 2ng.Furthermore,three types of case samples were selected,including trace DNA sample,to test the practical application of lyophilized reagents and liquid reagents were used in parallel for comparison.The results showed that DNATyperTM 30 lyophilized reagent had accurate typing results and the peak value was between 3000 and 20000 RFUs with good repeatability.The lyophilized reagent was validated to get a well-balanced STR typing result,and the sensitivity was 0.125 ng,while it showed good stability when stored at room temperature for over 1 month.Essentially,lyophilized reagents performed outstandingly in the detection of trace DNA samples,with a higher detection rate and higher peak height of loci compared to conventional reagents.Besides,compared to longer segment loci,lyophilized reagents had a higher detection rate for shorter segment loci.Overall,DNATyperTM 30 lyophilized reagent,which is easy to operate and has a high sensitivity,can significantly increase the loading quantity of DNA tamplate,and extend the storage time at room temperature,and meet the needs of DNA tracing testing,long-distance transportation and room temperature carrying at the same time.Therefore,lyophilized reagents have broad prospects in forensic examination and good practical application value.关键词
法医遗传学/冻干试剂/微量DNA/短串联重复序列/DNATyperTM 30试剂盒Key words
forensic genetics/lyophilized reagent/trace DNA/STR/DNATyperTM30 kit分类
政治法律引用本文复制引用
张哲,于浩,李晨佳,余政梁,吴俞衡,张广峰,李万水,白雪..DNATyperTM 30六色荧光STR复合扩增冻干试剂的验证[J].刑事技术,2023,48(6):616-622,7.基金项目
公安部科技强警基础工作专项(2021JC13) （2021JC13）
国家重点研发计划(2021YFC3300104) （2021YFC3300104）
