刑事技术2023，Vol.48Issue(6)：645-648,4.DOI:10.16467/j.1008-3650.2023.6017
DNA二代测序技术在30年陈旧骨骼检验中的应用
Application of Next Generation Sequencing in the Examination of 30 Years Old Skeleton
金鑫 1苏建富 2王亚萍3
作者信息
- 1. 海南省公安厅,海口 570311
- 2. 海南省东方市公安局,海南东方 572600
- 3. 新疆昌吉州公安局刑事科学技术研究所,新疆昌吉 831100
- 折叠
摘要
Abstract
DNA analysis by capillary electrophoresis with fluorescent short tandem repeat(STR)detection plays a dominant role in DNA testing of various cases,but for difficult biological samples,such as highly corrosive and degraded bones,it is not effective due to the small number of motifs STR detected.This paper describes the application of second-generation sequencing technology in DNA testing of difficult biological samples such as highly degraded bones in forensic science practice.In a murder case that had remained unsolved for 30 years,the traditional STR testing method did not yield effective STR locus typing,but a complete autosomal locus typing was obtained by second-generation sequencing using the Precision ID GlobalFilerTM NGS STR Panel v2 kit.However,through the second-generation sequencing method using Precision ID GlobalFilerTM NGS STR Panel v2 kit,the genotypes of 31 autosomal loci were obtained,which helped the case unit to identify the source of the body and provided key technical support for the investigation of the case.By analyzing the advantages of NGS technology for such highly degradable samples and exploring the consistency between the STR locus typing results of CE electrophoresis detection and NGS typing results,it is shown that NGS technology can overcome the analysis limitations of CE for such highly degradable samples,supplement the shortcomings of CE technology,and provide new methods and technique for forensic DNA testing and analysis of difficult biological samples.关键词
法医遗传学/短串联重复序列/二代测序技术/降解生物检材/骨骼Key words
forensic genetics/STR/next generation sequencing(NGS)/degradation biological samples/bones分类
社会科学引用本文复制引用
金鑫,苏建富,王亚萍..DNA二代测序技术在30年陈旧骨骼检验中的应用[J].刑事技术,2023,48(6):645-648,4.
