烟草科技2023，Vol.56Issue(12)：1-6,6.DOI:10.16135/j.issn1002-0861.2023.0136
dsRNA-endoPGs和dsRNA-RMK1介导的RNAi对烟草靶斑病的抑制作用
Inhibition effects of RNAi mediated by dsRNA-endoPGs and dsRNA-RMK1 on tobacco target spot disease
摘要
Abstract
To safely and effectively prevent and control tobacco target spot disease,endo polygalacturonases(endoPGs)and MAP kinases of the tobacco target spot pathogen(Rhizoctonia solani YC-9)were used as the targets to design and synthesize dsRNA-endoPGs and dsRNA-RMK1 by RNA interference technology.The control effects of dsRNA-endoPGs and dsRNA-RMK1 on tobacco target spot were analyzed by spraying on tobacco leaves in vitro and tobacco plants in the field.The results showed that 7 d after spraying dsRNA-endoPGs and dsRNA-RMK1 on tobacco leaves in vitro,the inhibition rates of tobacco target spot lesions were 62.10%and 60.00%,respectively.After spraying dsRNA-endoPGs or dsRNA-RMK1 on the leaves of tobacco plants in field trials once every 7 d and 3 times in total,the relative control efficacies on tobacco target spot 7,14,21 d after the first spray were all above 50%.关键词
烟草靶斑病/双链RNA/RNA干扰Key words
Tobacco target spot disease/Double-stranded RNA/RNA interference分类
农业科技引用本文复制引用
王妍,郭依,汪汉成,蔡刘体,安梦楠,张崇,吴元华..dsRNA-endoPGs和dsRNA-RMK1介导的RNAi对烟草靶斑病的抑制作用[J].烟草科技,2023,56(12):1-6,6.基金项目
中国烟草总公司科技重大专项项目"烟草主要叶斑类病害监测预警及绿色防控技术研究与应用"[110202001035(LS-04)]. （LS-04）
