烟草科技2023，Vol.56Issue(12)：7-13,7.DOI:10.16135/j.issn1002-0861.2023.0642
不同浓度吐温-80对烟草叶面微生物洗脱效果的影响
Effects of Tween-80 at different concentrations on elution of microorganisms from tobacco leaf surface
摘要
Abstract
To establish an efficient collection system for tobacco leaf surface microorganisms,high-secreting tobacco T.I.1068 was used as the research material.Solutions of Tween-80 at concentrations(volume fraction)of 0,2％,8％,10％,15％,20％,and 30％ were used to elute and collect leaf surface microorganisms.The effects of Tween-80 on the elution of leaf surface microorganisms from high-secreting tobacco were studied by plate culture and amplicon sequencing.The results are as follows:1)With the increase of Tween-80 concentration,the number of cultivable microorganisms on the plate first increased and then decreased.The bacteria colony density was the highest under the treatment of 15％ Tween-80,and that of fungi was the highest under the treatment of 10％ Tween-80.2)With increasing Tween-80 concentration,the total DNA concentration of the foliar microorganisms showed an increasing trend and reached a plateau at 20％ or above.3)16S rDNA amplicon sequencing analysis showed that the richness and evenness of bacterial species obtained by 20％ Tween-80 elution were higher than those of aqueous solutions,but the structure of the community of bacteria changed,and the abundance of bacterial families such as Xanthomonadaceae,Xanthomonadaceae,Sphingomonadaceae,Rhizobiaceae and Pseudomonadaceae increased significantly.4)Amplicon sequencing and ITS rDNA analysis showed that the richness of fungal species obtained by 20％ Tween-80 elution was lower than that of the aqueous solution,but the structure of the fungal community changed,and the abundance of Erysiphaceae and Phaffomycetaceae,the fungal families,increased significantly.In conclusion,Tween-80 could effectively improve the efficiency of microbial elution and the concentration of total microbial DNA,and had a positive effect on microbial culture and community structure and diversity analysis.关键词
烟草/叶面微生物/吐温-80/微生物收集/扩增子测序Key words
Tobacco/Foliar microorganism/Tween-80/Microorganism sampling/Amplicon sequencing分类
轻工纺织引用本文复制引用
张振远,滕环瑜,潘影,王召军,张洪映,闫筱筱,崔红..不同浓度吐温-80对烟草叶面微生物洗脱效果的影响[J].烟草科技,2023,56(12):7-13,7.基金项目
中国烟草总公司烤烟基因组计划和生物育种重大专项项目"烤烟叶面化学成分的分子调控和定向改良"[110202101005(JY-05)]. （JY-05）
