现代畜牧科技Issue(12)：19-22,4.DOI:10.19369/j.cnki.2095-9737.2023.12.005
水产病原肝肠孢虫核酸检测试剂盒效果测评
Evaluation of the Effect of Nucleic Acid Detection Kit for Aquatic Pathogenic Enterocytozoon hepatopenaei
摘要
Abstract
This study selected three nucleic acid extraction kits,namely Huikeyin,Market Brand A,and Market Brand B,as well as their corresponding PCR detection kits,to detect the rampant aquatic disease Enterocytozoon hepatopenaei in recent years.The nucleic acid concentration,purity,CV value extracted by the nucleic acid extraction kit were comprehensively evaluated,as well as the Ct value and fluctuation range of the RT-PCR detection kit.The results showed that the concentrations of nucleic acid extracted by different nucleic acid extraction kits from the liver,pancreas,and muscle tissues of Penaeus vannamei were as follows:Huikoin＞B＞A;The EHP pathogen concentration obtained from nucleic acid samples obtained from different extraction kits through different qPCR tests is as follows:Huikeyin≥A＞B;The Huikyin nucleic acid extraction kit and qPCR detection kit have better performance and stability compared to the A and B kits.关键词
肝肠孢虫/核酸提取/qPCR核酸检测试剂盒/南美白对虾Key words
Enterocytozoon hepatopenaei/nucleic acid extraction/qPCR nucleic acid detection kit/penaeus vannamei分类
农业科技引用本文复制引用
李丰,俞婷,张健玲,王凯,邹祺琪..水产病原肝肠孢虫核酸检测试剂盒效果测评[J].现代畜牧科技,2023,(12):19-22,4.基金项目
2020年度广东省科技专项资金("大专项+任务清单")项目(2020A02015) （"大专项+任务清单"）
2021年广东省乡村振兴战略专项资金("大专项+任务清单")项目(江科[2021]183号) （"大专项+任务清单"）
