水产病原肝肠孢虫核酸检测试剂盒效果测评OA

This study selected three nucleic acid extraction kits,namely Huikeyin,Market Brand A,and Market Brand B,as well as their corresponding PCR detection kits,to detect the rampant aquatic disease Enterocytozoon hepatopenaei in recent years.The nucleic acid concentration,purity,CV value extracted by the nucleic acid extraction kit were comprehensively evaluated,as well as the Ct value and fluctuation range of the RT-PCR detection kit.The results showed that the concentrations of nucleic acid extracted by different nucleic acid extraction kits from the liver,pancreas,and muscle tissues of Penaeus vannamei were as follows:Huikoin＞B＞A;The EHP pathogen concentration obtained from nucleic acid samples obtained from different extraction kits through different qPCR tests is as follows:Huikeyin≥A＞B;The Huikyin nucleic acid extraction kit and qPCR detection kit have better performance and stability compared to the A and B kits.