现代畜牧科技Issue(12)：32-34,3.DOI:10.19369/j.cnki.2095-9737.2023.12.008
布鲁氏菌Rev.1株接种不同条件制备TSA培养基验证试验
Verification Test of Preparing TSA Medium by Inoculating Brucella Rev.1 Strain under Different Conditions
吴梅花 1狄栋栋 1赵丹彤 1白白音宝力高 1侯振宇1
作者信息
- 1. 金宇保灵生物药品有限公司,内蒙古 呼和浩特 010111
- 折叠
摘要
Abstract
Brucellosis is one of the zoonotic diseases of animal origin that seriously threatens human health.The prevention and control of brucellosis in animals is mainly based on prevention.At present,there are S2,A19 and M5 vaccines for brucellosis in China,and S19 and Rev.1 vaccines in the world,among which M5 and Rev.1 strains of sheep are the least stable.TSA is Tryptone soy agar medium(also known as Tryptone soy agar medium),a commercial medium that is suitable for the growth of Brucella Rev.1 strain.After TSA medium plate with different agar concentration was placed under different conditions(temperature and time),Brucella Rev.1 strain was inoculated.After 96 h culture,colony growth was observed and colony crystal violet staining was performed.Through comparative analysis,it is found that the humidity of TSA medium plate is an important factor affecting the accuracy of variation test results.At the same time,the preparation method of TSA medium for Brucella Rev.1 strain was clarified.关键词
布鲁氏菌Rev.1株/琼脂浓度/温度和时间/变异率Key words
Brucella Rev.1/agar concentration/temperature and time/variation rate分类
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吴梅花,狄栋栋,赵丹彤,白白音宝力高,侯振宇..布鲁氏菌Rev.1株接种不同条件制备TSA培养基验证试验[J].现代畜牧科技,2023,(12):32-34,3.
