中国当代儿科杂志2023，Vol.25Issue(12)：1227-1233,7.DOI:10.7499/j.issn.1008-8830.2309080
CHI3L1在川崎病样血管炎小鼠模型冠状动脉损伤中的作用及机制研究
Role and mechanisms of CHI3L1 in coronary artery lesions in a mouse model of Kawasaki disease-like vasculitis
摘要
Abstract
Objective To explore the role and potential mechanisms of chitinase-3-like protein 1(CHI3L1)in coronary artery lesions in a mouse model of Kawasaki disease(KD)-like vasculitis.Methods Four-week-old male SPF-grade C57BL/6 mice were randomly divided into a control group and a model group,with 10 mice in each group.The model group mice were intraperitoneally injected with 0.5 mL of lactobacillus casei cell wall extract(LCWE)to establish a mouse model of KD-like vasculitis,while the control group mice were injected with an equal volume of normal saline.The general conditions of the mice were observed on the 3rd,7th,and 14th day after injection.Changes in coronary artery tissue pathology were observed using hematoxylin-eosin staining.The level of CHI3L1 in mouse serum was measured by enzyme-linked immunosorbent assay.Immunofluorescence staining was used to detect the expression and localization of CHI3L1,von Willebrand factor(vWF),and α-smooth muscle actin(α-SMA)in coronary artery tissue.Western blot analysis was used to detect the expression of CHI3L1,vWF,vascular endothelial cadherin(VE cadherin),Caspase-3,B cell lymphoma-2(Bcl-2),Bcl-2 associated X protein(Bax),nuclear factor κB(NF-κB),and phosphorylated NF-κB(p-NF-κB)in coronary artery tissue.Results The serum level of CHI3L1 in the model group was significantly higher than that in the control group(P<0.05).Compared to the control group,the expression of CHI3L1 in the coronary artery tissue was higher,while the expression of vWF was lower in the model group.The relative expression levels of CHI3L1,Bax,Caspase-3,NF-κB,and p-NF-κB were significantly higher in the model group than in the control group(P<0.05).The relative expression levels of vWF,VE cadherin,and Bcl-2 were lower in the model group than in the control group(P<0.05).Conclusions In the LCWE-induced mouse model of KD-like vasculitis,the expression levels of CHI3L1 in serum and coronary arteries increase,and it may play a role in coronary artery lesions through endothelial cell apoptosis mediated by inflammatory reactions.关键词
川崎病/壳多糖酶3样蛋白1/血管炎/冠状动脉损伤/细胞凋亡/小鼠Key words
Kawasaki disease/Chitinase-3-like protein 1/Vasculitis/Coronary artery lesion/Apoptosis/Mouse引用本文复制引用
曹越,高帅,罗刚,赵水炎,唐雅琪,杜占慧,泮思林..CHI3L1在川崎病样血管炎小鼠模型冠状动脉损伤中的作用及机制研究[J].中国当代儿科杂志,2023,25(12):1227-1233,7.基金项目
泰山学者工程资助(2018). （2018）
