中国动脉硬化杂志2023，Vol.31Issue(11)：929-937,9.DOI:10.20039/j.cnki.1007-3949.2023.11.002
剪切修复基因D通过下调mTOR/LOX-1抑制ox-LDL诱导的人脐静脉平滑肌细胞增殖
Xeroderma pigmentosum group D gene inhibits the proliferation of human umbilical vein smooth muscle cell induced by ox-LDLvia down-regulating mTOR/LOX-1 pathway
摘要
Abstract
Aim To investigate the mechanism and signal pathways of xeroderma pigmentosum group D(XPD)gene on the proliferation of human umbilical vein smooth muscle cell(HUVSMC)induced by ox-LDL.Methods HUVSMCs were transfected with the plasmids of pEGFP-N2/XPD using Lipofectamine 2000,and subsequently silent mTOR gene.MTT and EdU assay was used to detect the cell proliferation.Flow cytometry was used to examine the cell apoptosis.The expression of XPD,lectin-like oxidized low-density lipoprotein receptor 1(LOX-1),mTOR,phospho-mTOR,Bcl-2 and Bax was measured by Western blot.Results The expression of XPD and Bax protein was down-regulated in ox-LDL group(P＜0.05),while the expression of LOX-1,mTOR,Bcl-2 protein and the ratio of Bcl-2/Bax was significantly up-regulated(P＜O.05),compared with control group.Cell proliferation of ox-LDL group increased ob-viously(P＜0.05).After transfected with the pEGFP-N2/XPD plasmid,the expression of Bax was significantly up-regu-lated,while the expression of LOX-1,mTOR,Bcl-2 and the ratio of Bcl-2/Bax were significantly down-regulated(P＜0.05).Flow cytometry showed that overexpression of XPD increased the apoptosis rate of HUVSMC(P＜0.05).MTT and BdU showed that cell proliferation of pEGFP-N2/XPD group reduced compared with control group(P＜0.05).Com-pared with control group,the expression of LOX-1 was significantly down-regulated in siRNA mTOR group(P＜0.05).Conclusion XPD can inhibit HUVSMC proliferation and promote its apoptosis,and reduce the effect of ox-LDL promoting proliferation of HUVSMC via the mTOR/LOX-1 pathway.XPD may be the target of treatment of atherosclerosis.关键词
剪切修复基因/氧化型低密度脂蛋白/人脐静脉平滑肌细胞/动脉粥样硬化/mTORKey words
xeroderm pigmentosum group D gene/ox-LDL/human umbilical vein smooth muscle cell/ather-osclerosis/mTOR分类
医药卫生引用本文复制引用
余卫英,夏子荣,李青,夏珍,李菊香..剪切修复基因D通过下调mTOR/LOX-1抑制ox-LDL诱导的人脐静脉平滑肌细胞增殖[J].中国动脉硬化杂志,2023,31(11):929-937,9.基金项目
江西省自然科学基金资助项目(2019GZY0254) （2019GZY0254）
江西省卫生健康委科技计划(20204238) （20204238）
