中国动脉硬化杂志2023，Vol.31Issue(11)：958-968,11.DOI:10.20039/j.cnki.1007-3949.2023.11.005
外周血白细胞PTK7表达水平与冠心病的相关性及其诊断价值
Impacts of protein tyrosine kinase 7 expression in peripheral leukocytes on the devel-opment of coronary heart disease and its diagnostic value
摘要
Abstract
Aim To explore the correlation between protein tyrosine kinase 7(PTK7)and coronary heart disease(CHD)and its diagnostic value.Methods Target genes were obtained through the Gene Expression Omnibus(GEO)database.StataSE15 was used to find the total standardized mean difference(SMD)of PTK7 and plot the sum-mary receiver operating characteristic(SROC)curve.Next,reverse transcription quantitative polymerase chain reaction was used to verify the expression of PTK7 in CHD and non-CHD population samples and search for CHD-related single-cell RNA sequencing data from GEO to analyze the expression of PTK7 in different cells.The upstream transcription factor(TF)of PTK7 was predicted by the Cistrome Data Browser database.Moreover,enrichment analysis of the gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)were performed on the differentially co-expressed genes.Results By calculating the SMD of PTK7,it was found that PTK7 was highly expressed in the peripheral blood leukocytes(PBL)of patients with CHD(total SMD=0.81,95％confidence interval=0.17～1.45).The population sample vali-dation confirmed the above results.When SROC was plotted,the area under the curve(AUC)was 0.79,indicating that PTK7 has the ability to distinguish between CHD and non-CHD.The single-cell RNA sequencing results showed that the expression ratio of PTK7 was relatively low in different cells of normal peripheral blood.In addition,potential upstream TFs of PTK7 were predicted through the ChIP-seq database,where it was found that IRAKI,SNAI2 may be positive up-stream TFs of PTK7,and EP300,NIPBL may be negative upstream TFs of PTK7.Conclusion Highly expressed PTK7 in PBL is positively correlated with the pathogenesis of CHD,demonstrating that PTK7 has definite diagnostic value on CHD.关键词
冠心病/蛋白酪氨酸激酶7/单细胞RNA测序分析/反转录定量PCR/生物信息学Key words
coronary heart disease/protein tyrosine kinase 7/single-cell RNA sequencing/reverse transcrip-tion quantitative polymerase chain reaction(RT-qPCR)/bioinformatics分类
医药卫生引用本文复制引用
杨英,张义炜,陈宁园,黄玲,潘尚领..外周血白细胞PTK7表达水平与冠心病的相关性及其诊断价值[J].中国动脉硬化杂志,2023,31(11):958-968,11.基金项目
国家自然科学基金项目(81960266) （81960266）
广西自然科学基金面上项目(2022JJA140739) （2022JJA140739）
广西医科大学长寿与老年相关疾病教育部重点实验室开放课题(KLLAD201902) （KLLAD201902）
