摘要
Abstract
Objective To investigate the regulatory role of miR-210 in the pathogenesis of spinal cord injury(SCI)-induced osteoporosis.Methods Thirty patients with osteoporosis after SCI(treatment group),elderly patients with osteoporosis(control group)and healthy subjects(normal group)were included.Blood samples were collected and ELISA was used to detect serum levels of interleukin-1 β(IL-1 β),tumor necrosis factor-α(TNF-α)and bone alkaline phosphatase(BLAP).The expression level of miR-210 was detected by real-time polymerase chain reaction.SCI model was constructed by spinal cord stripping method.Twenty-four SD rats were randomly divided into sham-operation,control(SCI modeling+tail vein injection of 20 nmol·kg-1 agomiR-NC)and experimental(SCI modeling+tail vein injection of 20 nmol·kg-1 agomiR-210)groups.Three groups were administered twice a week for 28 days.Basso,Beattie,Bresnahan locomotor rating scale(BBB score)and serum IL-1β,TNF-αand BLAP levels were compared among the groups.Results In the clinical trial,IL-1β levels of normal,control and treatment groups were(103.06±13.25),(110.64±14.46)and(318.63±32.60)pg·mL-1;TNF-α levels were(44.01±6.92),(57.00±7.12)and(165.48±24.86)pg·mL-1;miR-210 expression levels were 1.00±0.17,1.35±0.40 and 0.20±0.05,respectively.The treatment group showed significant differences compared to the control group in all indicators(all P＜0.05).In the rat experiment,the IL-1β levels were(92.50±11.59),(621.48±48.36)and(288.55±61.46)pg·mL-1;the TNF-α levels were(37.22±3.13),(259.79±41.76)and(159.79±12.27)pg·mL-1;the BLAP levels were(84.51±11.44),(301.59±39.53)and(203.98±20.24)U·L-1;the BBB scores on day 28 were(21.00±0.00),(12.83±1.40)and(16.66±2.48)points for the sham-operation,control and experimental groups,respectively.Conclusion Overexpression of miR-210 can alleviate osteoporosis caused by SCI through reducing levels of inflammatory cytokines.关键词
miR-210/脊髓损伤/骨质疏松/炎性因子Key words
miR-210/spinal cord injury/osteoporosis/inflammatory cytokines分类
医药卫生
