中国临床药理学杂志2023，Vol.39Issue(23)：3409-3413,5.DOI:10.13699/j.cnki.1001-6821.2023.23.015
白芥子对类风湿性关节炎滑膜细胞增殖、凋亡的影响
Effects of semen brassicae albae on proliferation and apoptosis of rheumatoid arthritis synoviocytes
摘要
Abstract
Objective To investigate the effects of semen brassicae albae on the proliferation and apoptosis of rheumatoid arthritis(RA)synoviocytes,and to analyze the related mechanisms.Methods Logarithmic RA fibroblastic synovial cells(RA-FLS)were randomly divided into blank group,experimental-L,M,H groups and positive control group.Blank group did not make any treatment.Experimental-L,M,H groups were cultured using DMEM medium containing 20,40,80 μg·mL-1 of semen brassicae albae extract,respectively.Positive control group was cultured at DMEM medium containing 5 mg·L-1 of Tripterygium glycosides.Five groups were cultured for 48 h.The survival rates of RA-FLS cells were detected by CCK-8 method,the apoptosis rates of RA-FLS cells were detected by Annexin V-FITC/PI double staining method,and the expression levels of proliferation and apoptosis-related proteins were detected by Western blotting.Results The cell survival rates of experimental-M,-H groups,blank group and positive control group were(71.35±10.86)％,(58.56±17.92)％,(99.12±0.38)％and(56.21±15.11)％;the apoptosis rates were(28.98±4.35)％,(39.12±5.85)％,(8.96±1.36)％and(41.21±6.01)％;the expression levels of PCNA protein were 0.87±0.13,0.50±0.08,1.30±0.19 and 0.49±0.09;the expression levels of Bel-2 protein were 1.20±0.14,1.35±0.19,0.45±0.06 and 1.31±0.21;the expression levels of Bax protein were 0.65±0.09,0.27±0.04,1.14±0.15 and 0.29±0.06,respectively.Compared with blank group,the differences of above indexes of experimental-M,-H groups and positive control group were statistically significant(all P＜0.05).Conclusion Semen brassicae albae can inhibit the proliferation of RA-FLS cells and induce cell apoptosis,and its mechanism may be related to regulating the expression of proliferation and apoptosis related proteins.关键词
白芥子/类风湿性关节炎/滑膜细胞/增殖/凋亡Key words
semen brassicae albae/rheumatoid arthritis/synonial cell/proliferation/apoptosis分类
医药卫生引用本文复制引用
钱晓晋,彭仁亮,倪晨波..白芥子对类风湿性关节炎滑膜细胞增殖、凋亡的影响[J].中国临床药理学杂志,2023,39(23):3409-3413,5.基金项目
福建省自然科学基金资助项目(2018J05149) （2018J05149）
