中国临床药理学杂志2023，Vol.39Issue(23)：3469-3474,6.DOI:10.13699/j.cnki.1001-6821.2023.23.027
用HPLC-MS/MS法测定儿童微量血浆中多种治疗性药物的浓度
Determination of multifarious drugs in trace plasma of children by HPLC-MS/MS
摘要
Abstract
Objective To develop a high performance liquid chromatography-tandem mass spectrometry method for simultaneous determination of multifarious drugs in human plasma for therapeutic drug monitoring in children.Methods The proteins in 10 μL plasma samples were precipitated by methanol.The chromatographic conditions were as following:chromatographic column:Phenomenex Kinetex EVO C18 column(50 × 2.1 mm,2.6 μm),mobile phase:water(1.0 × 10-5 formic acid)-methanol,flow rate:0.45 mL·min-1,The column temperature:45 ℃.The electrospray ionization source was used and the mass detection was performed using dynamic multiple reaction monitoring mode with ionization polarity switching.Results In plasma samples,methotrexate(linear range 31.25-1 000.00 ng·mL-1),levetiracetam and 10,11-dihydro-10-hydroxycarbazepine(linear range 1.56-50.00 μg·mL-1),lamotrigine and carbamazepine(linear range 0.78-25.00 μg·mL-1),showed good linear relationships under this method(all R2＞0.99).The specificity,precision and accuracy values for intra-and inter-assays,matrix effect,stability all met the requirements.This method was applied to the therapeutic drug monitoring in 498 pediatric patients.Conclusion The method is simple,sensitive,rapid and suitable for children therapeutic drug monitoring.关键词
甲氨蝶呤/抗癫痫药物/高效液相色谱串联质谱法/治疗药物监测/儿童Key words
methotrexate/multifarious antiepileptic drug/high performance liquid chromatography-tandem mass spectrometry/therapeutic drug monitoring/children分类
医药卫生引用本文复制引用
秦亚彬,张古英,孙玉林,赵德运..用HPLC-MS/MS法测定儿童微量血浆中多种治疗性药物的浓度[J].中国临床药理学杂志,2023,39(23):3469-3474,6.基金项目
河北省卫健委医学科学研究课题基金资助项目(20190795) （20190795）
