| 注册
首页|期刊导航|中国农业科学|小麦粒重相关性状的QTL定位及分子标记的开发

小麦粒重相关性状的QTL定位及分子标记的开发

张泽源 宋全昊 简俊涛 孙道杰 王兴荣 李玥 赵文莎 顾晶晶 张傲琰 张海龙 宋鹏博 吴建辉 张传量

中国农业科学2023,Vol.56Issue(21):4137-4149,中插1-中插13,26.
中国农业科学2023,Vol.56Issue(21):4137-4149,中插1-中插13,26.DOI:10.3864/j.issn.0578-1752.2023.21.001

小麦粒重相关性状的QTL定位及分子标记的开发

QTL Mapping and Molecular Marker Development of Traits Related to Grain Weight in Wheat

张泽源 1宋全昊 2简俊涛 3孙道杰 1王兴荣 4李玥 4赵文莎 1顾晶晶 5张傲琰 1张海龙 1宋鹏博 1吴建辉 1张传量1

作者信息

  • 1. 西北农林科技大学农学院,陕西杨凌 712100
  • 2. 驻马店市农业科学院,河南驻马店 463000
  • 3. 南阳市农业科学院,河南南阳 473000
  • 4. 甘肃省农业科学院作物研究所,兰州 730000
  • 5. 洛阳市农林科学院,河南洛阳 471023
  • 折叠

摘要

Abstract

[Objective]The yield of wheat,the second-highest-yielding food product in the world,has a major impact by grain weight.This research used materials from a recombinant inbred line(RIL)population derived from Heshangtou(HST)and Longchun 23(LC23).Based on 55K SNP genotype data,QTL mapping was performed for traits related to grain weight of wheat,and co-segregation markers of major grain length QTL were developed and verified to provide reference for molecular marker assisted selection breeding.[Method]The wheat 55K SNP microarray was used to genotype parents and RIL populations,and a high density genetic linkage map was constructed,and its correlation with Chinese spring reference genome IWGSC RefSeq v1.0 was analyzed.QTL mapping of traits related to grain weight in multiple environments based on inclusive composite interval mapping method.The analysis of variance of major effect QTLs were performed to judge the additive interaction effect among different QTLs,and to analyse its effect on traits related to grain weight.At the same time,the corresponding kompetitive allele specific PCR marker was developed according to the closely linked SNP loci of major QTL for grain length,and verified in 242 wheat accessions worldwide.[Result]In this study,a high density genetic map of Heshangtou/Longchun 23 RIL population was constructed,with full length 4 543 cM,including 22 linkage groups,covering 21 chromosomes of wheat,and the average genetic distance was 1.7 cM.There was a significant correlation between genetic map and physical map,and the Pearson correlation coefficient were 0.77-0.99(P<0.001).A total of 51 QTLs related to grain weight were detected,among them,4 stable major QTLs were found in multi-environments(three or more environments)and distributed on 2D,5A,6B and 7D chromosomes.According to the physical interval and functional markers,it is inferred that stable major QTLs Qtkw.nwafu-2D.1 and Qtkw.nwafu-7D are photoperiod gene Ppd-D1 and flowering gene FT-D1,respectively.The analysis of variance shows that there is a significant interaction between them.The favorite alleles polymerization of Qtkw.nwafu-2D.1 and Qtkw.nwafu-7D can significantly increase thousand grain weight and grain width of wheat.In addition,the corresponding KASP molecular detection marker AX-111067709 was developed based on the co-segregated SNP of the major locus Qgl.nwafu-5A for grain length,which was significantly correlated with grain length and grain weight traits in a diversity panel comprising of 242 wheat accessions,and could increase grain length by 3.33%to 4.59%and grain weight 5.70%to 10.35%in different environments(P<0.001).[Conclusion]There are several genetic loci that affect traits linked to grain weight in Heshangtou(HST)and Longchun 23(LC23),and Qtkw.nwafu-2D.1 and Qtkw.nwafu-7D dramatically increased thousand grain weight and grain width through additive interaction effects.Qgl.nwafu-5A is significantly correlated with grain weight and grain length,and its co-segregated molecular marker AX-11106770 can be used in molecular marker assisted selection breeding.

关键词

小麦/千粒重/QTL/KASP标记/分子标记辅助选择育种

Key words

wheat/thousand-grain weight/QTL/KASP marker/molecular marker-assisted selection breeding

引用本文复制引用

张泽源,宋全昊,简俊涛,孙道杰,王兴荣,李玥,赵文莎,顾晶晶,张傲琰,张海龙,宋鹏博,吴建辉,张传量..小麦粒重相关性状的QTL定位及分子标记的开发[J].中国农业科学,2023,56(21):4137-4149,中插1-中插13,26.

基金项目

陕西省"两链"融合重点专项(2023KXJ-011) (2023KXJ-011)

中国农业科学

OA北大核心CSCDCSTPCD

0578-1752

访问量0
|
下载量0
段落导航相关论文