中国农业科学2023，Vol.56Issue(21)：4163-4174,中插16-中插25,22.DOI:10.3864/j.issn.0578-1752.2023.21.003
糜子核心种质成株期抗旱性鉴定评价与抗旱种质筛选
Identification and Evaluation of Drought Tolerance and Screening of Drought-Tolerant Germplasm for Core Germplasms in Proso Millet at Adult Stage
摘要
Abstract
[Objective]Drought is one of the major constraints influencing on the growth,development,and yield of proso millet.To screen excellent drought-tolerant accessions and identification indicators will promote the drought-tolerant varieties breeding and drought tolerance molecular mechanisms analysis of proso millet.[Method]In this study,200 core germplasm accessions of proso millet were used to identify field drought tolerance at adult stage in 2021-2022 at Dunhuang,Gansu province,with two treatments of normal irrigation and drought stress.Eleven morphological indicators,such as leaf area(LA),main stem diameter(MSD),number of main stem nodes(NMSN),main panicle length(MPL),peduncle length(PL),plant height(PH),straw weight per plant(SWPP),panicle weight per plant(PWPP),grain weight per plant(GWPP),thousand grain weight(TGW),and yield per plot(YPP)were determined.Comprehensive drought tolerance coefficient(CDTC value),drought resistance index(DRI value),and drought resistance comprehensive evaluation value(D value)were combined to identify the drought tolerance of proso millet at adult stage.[Result]There were significant differences in all eleven indexes among different accessions under different water treatments,including leaf area,main stem diameter,number of main stem nodes,main panicle length,peduncle length,plant height,straw weight per plant,panicle weight per plant,grain weight per plant,thousand grain weight,and yield per plot.The growth of proso millet was inhibited under drought stress treatments.Compared with the normal irrigation treatments,all the eleven indexes under drought treatments were significantly reduced,and yield per plot was more sensitive to drought treatments.The correlation analysis found that certain degrees of correlation existed among the drought tolerance coefficients of all traits,the correlation between panicle weight per plant and grain weight per plant was strongest,with a correlation coefficient of 0.943.Eleven evaluation indexes were converted into six comprehensive indexes by principal component analysis,with a cumulative variance contribution of 80.667%.The drought tolerance ranking of proso millet accessions based on CDTC value,DRI value and D value was generally consistent.The 200 proso millet accessions were classified into four categories according to D value cluster analysis,10 of cluster Ⅰ were highly drought tolerant,70 of cluster Ⅱ were drought tolerant,81 of cluster Ⅲ were drought sensitive,and 39 of cluster Ⅳ were highly drought sensitive.Plant height,grain weight per plant,panicle weight per plant and main panicle length were highly correlated with D value,with correlation coefficients of 0.756,0.697,0.696 and 0.679,respectively.The regression equation for the drought tolerance evaluation was constructed by stepwise regression analysis:Y=-1.509+0.362X1+0.174X2+0.349X3+0.389X4+0.307X5+ 0.251X6+0.218X7.[Conclusion]The drought resistance comprehensive evaluation value method could be suitable for evaluating the drought resistance of proso millet at adult stage.Ten accessions with highly drought tolerance,such as Balinzuogedashu(00000525),Gaotaiwumizi(00002677)and Minlehongmizi(00002687).Plant height,panicle weight per plant and main panicle length could be used as primary evaluation indexes for drought tolerance of proso millet at adult stage.关键词
糜子/大田成株期/抗旱鉴定/抗旱种质筛选Key words
proso millet/adult stage in the field/drought tolerance identification/drought tolerant germplasm screening引用本文复制引用
王倩,刘敏轩,董孔军,薛亚鹏,刘少雄,王若楠,杨佳琪,陆平,王瑞云,杨天育..糜子核心种质成株期抗旱性鉴定评价与抗旱种质筛选[J].中国农业科学,2023,56(21):4163-4174,中插16-中插25,22.基金项目
国家现代农业产业技术体系建设专项(CARS-06-14.5-A2)、农作物种质资源保护与利用专项(2022NWB036-06)、中国农业科学院科技创新工程 （CARS-06-14.5-A2）
