中国农业科学2023，Vol.56Issue(22)：4441-4452,12.DOI:10.3864/j.issn.0578-1752.2023.22.007
基于稻瘟病菌小种变化的吉林省主要粳稻品种抗性评价及利用价值分析
Evaluation of Resistance and Analysis of Utilization Value of the Major Japonica Rice Varieties in Jilin Province Based on the Physiological Race Variation of Magnaporthe oryzae
摘要
Abstract
[Objective]Rice blast poses a serious threat to the safe production of rice in Jilin Province,China.Breeding and utilizing resistant varieties are the most economical,safe,and effective measures to control rice blast.On the basis of clarifying the physiological race forms,distribution,and pathogenicity of Magnaporthe oryzae,the resistance evaluation to rice blast and variety combination utilization value analysis for major japonica rice varieties in Jilin Province were carried out.It will provide a theoretical basis for rational variety deployment and the efficient utilization of resistant varieties.[Method]In the autumn of 2021,62 dominant single spore strains were selected from 115 strains of M.oryzae collected and isolated from the main rice-growing areas of Jilin Province.These strains were then subjected to physiological race identification and pathogenicity analysis using seven Chinese differential varieties(CDVs).To assess the resistance of primary japonica rice varieties in Jilin Province,single pathogen inoculation at the seedling stage and field-induced resistance evaluation at various locations were carried out.Utilizing the results acquired from both the seedling stage and field identification,the resistance association coefficient(RAC)and virulence association coefficient(VAC)were used to analyze the disease resistance effect of various combinations of varieties.[Result]According to the resistance phenotype of different varieties(CDVs),62 M.oryzae strains could be divided into 7 groups and 22 physiological races.The dominant physiological groups were ZG and ZA,with frequencies of 35.48%and 32.26%,respectively.The dominant physiological races were ZG1,ZA1,and ZA17,with frequencies of 35.48%,9.68%,and 8.06%,respectively.The moderately virulent races with a pathogenicity rate of over 40%were ZA49,ZA1,ZB15,ZB23,and ZC15.The results of single-strain inoculation at the seedling stage of the main japonica rice varieties in Jilin Province showed that 48.89%of the tested rice varieties had a seedling blast resistance frequency above 80%.According to the field evaluation of panicle blast resistance,14 varieties demonstrated moderately resistant(MR),accounting for 15.56%of the evaluated varieties.Additionally,27 varieties exhibited moderately susceptible(MS),representing 30%of the evaluated varieties.Furthermore,35 varieties were classified as susceptible(S),making up 38.88%of the evaluated varieties.Finally,14 varieties exhibited highly susceptible(HS),representing 15.56%of the total.The analysis of disease resistance through the combinations of varieties revealed that the combinations simultaneous presence of a high RAC and low VAC have a good application prospect.[Conclusion]The physiological race structure of the 62 M.oryzae strains in Jilin Province is complex and diverse,with different dominant physiological races in different rice-growing regions.Additionally,the major japonica rice varieties in Jilin Province exhibit strong resistance and a broad resistance spectrum against the rice blast.Moreover,some combinations of varieties possess significant application value.关键词
稻瘟病/水稻品种/抗性评价/稻瘟病菌/生理小种Key words
rice blast/rice variety/resistance evaluation/Magnaporthe oryzae/physiological race引用本文复制引用
李莉,孙玲,张金花,邹晓威,孙辉,任金平,姜兆远,刘晓梅..基于稻瘟病菌小种变化的吉林省主要粳稻品种抗性评价及利用价值分析[J].中国农业科学,2023,56(22):4441-4452,12.基金项目
吉林省科技发展计划重点项目(20210202111NC)、国家农作物品种试验(抗性鉴定)、吉林省农业农村厅-吉林省主要农作物品种审定实验检测、鉴定 （20210202111NC）
