中国农业科学2023，Vol.56Issue(22)：4506-4522,中插1-中插16,33.DOI:10.3864/j.issn.0578-1752.2023.22.012
基于转录组和WGCNA筛选丝瓜果长和果径发育调控相关基因
Screening Regulatory Genes Related to Luffa Fruit Length and Diameter Development Based on Transcriptome and WGCNA
摘要
Abstract
[Objective]The aim of this study was to identify the co expression modules of luffa fruit length and diameter development and to screen key regulatory genes,so as to provide the theoretical basis for subsequent research on the molecular mechanism of fruit shape control in luffa.[Method]The luffa fruits in 9 fruit development stages(2 days before anthesis,and 0,2,4,6,8,10,15,and 20 days after anthesis)were applied as research materials.The fruit length and diameter of each stage were measured.The WGCNA method was used to jointly analyze transcriptome and fruit length and diameter data,to identify co-expressed gene modules of fruit length and diameter development,and to screen out key regulatory genes.[Result]A total of 14 co expression modules were identified by WGCNA,among which two modules(Turquoise and Lightpink4)were significantly correlated with fruit length and diameter(absolute value of correlation coefficient=0.9);Turquoise module was significantly positively correlated,while Lightpink4 module was significantly negatively correlated.KEGG enrichment analysis found that the Turquoise module was significantly enriched in endocytosis and phenylpropanoid biosynthesis pathways,which were closely related to fruit enlargement and growth regulation,and could be used as a key gene module for studying fruit length and diameter in luffa.According to the connectivity and functional annotation of genes in Turquoise module,ten key regulatory genes were screened,including xyloglucan endotransglucosylase/hydrolase gene XTH23,actin-depolymerizing factor gene ADF2,chaperone protein gene DnaJ10,expansin gene(EXPA1,EXPA4 and EXLA5),kinesin gene kinesin-13A,auxin response genes SAUR21,and Aux/IAA11.The RT-qPCR results showed that the expression levels of ten regulatory genes significantly increased after the fruit entered the rapid growth period(8 day after anthesis),with an increase of 2-50 times approximately.Through constructing a gene interaction network,it was found that some candidate genes interacted with the WRKY,bHLH,and HSF transcription factor families.[Conclusion]The Turquoise module,an important co expression module of luffa fruit length and diameter was obtained,and ten potential candidate genes for luffa fruit shape control were screened.It was found that luffa fruit length and diameter development regulation mainly involved the processes of cell wall reconstruction,cell development and differentiation,and auxin regulation.关键词
转录组/WGCNA/丝瓜/果长/果径/调控基因Key words
transcriptome/WGCNA/Luffa cylindrica L./fruit length/fruit diameter/regulatory genes引用本文复制引用
陈敏氡,王彬,刘建汀,李永平,白昌辉,叶新如,裘波音,温庆放,朱海生..基于转录组和WGCNA筛选丝瓜果长和果径发育调控相关基因[J].中国农业科学,2023,56(22):4506-4522,中插1-中插16,33.基金项目
福建省自然科学基金(2021J01494)、福建省农业科学院英才项目(YC2021004)、福建省农业科学院科技创新团队项目(CXTD2021003-1)、国家大宗蔬菜产业技术体系福州综合试验站项目(CARS-23-G51)、福建省种业创新与产业化工程项目(zycxny2021009)、福建省农业高质量发展超越"5511"协同创新工程项目(XTCXGC2021003) （2021J01494）
