中国农业科学2023，Vol.56Issue(22)：4532-4551,20.DOI:10.3864/j.issn.0578-1752.2023.22.014
饲粮纤维水平对猪肠道屏障功能、结肠微生物及代谢产物的影响
Effects of Dietary Fiber Level on Intestinal Barrier Function,Colonic Microbiota and Metabolites in Pigs
摘要
Abstract
[Objective]Adding fiber raw materials is one of the effective methods to reduce the feed costs.The aim of this study was to investigate the effects of different fiber level diets on the intestinal health of Mashen(MS)pig and Duroc×Landrace×Large(DLY)pig,so as to provide the basis for the rational use of fiber.[Method]In this study,80 MS pigs and 80 DLY pigs with an initial body weight of(20±0.5)kg were used as test objects(half of the sows and half of the boars).MS and DLY pigs were divided into four groups each assigned to different diets,with five replicates per treatment and four pigs per replicate.When 0%,9.35%,18.64%and 28.03%soybean hulls were added to the corn soybean meal basal diet,the NDF content was 9%(9N),13.5%(13.5N),18%(18N)and 22.5%(22.5N),respectively.The test lasted for 30 days.[Result]For MS pigs,the content of IL-10 in ileum of 18N group and TNF-α in cecum of 13.5N and 22.5N groups was significantly decreased(P＜0.05).The number of goblet cells in jejunum,ileum and caecum was increased with the increase of fiber level.In colon,the number of goblet cells and the expression level of MUC2 were significantly increased in 13.5N and 18N groups(P＜0.05).The expressions of Claudin-2,Occludin,E-cadherin,ZO-1,and ZO-2 of 18N and 22.5N groups were increased significantly(P＜0.05).The abundance of Escherichia-Shigella in 13.5N,18N and 22.5N groups were decreased significantly(P＜0.05),while the abundance of Lactobacillus in 13.5N group,Prevoteaceae_NK3B31_group in 18N group and Methanobrevibacter in 22.5N group were increased significantly(P＜0.05).Palmitic acid,stearic acid,lauric acid and capric acid in 13.5N,18N and 22.5N groups were significantly increased(P＜0.05),while lithocholic acid and cholic acid were significantly decreased(P＜0.05).Differential metabolites were mainly concentrated in the pathways related to lipid metabolism and carbohydrate digestion and absorption.For DLY pigs,the TNF-α content in cecum of 13.5N group was significantly increased(P＜0.05).The number of goblet cells in each intestinal segment of 13.5N,18N and 22.5N groups was significantly higher than those of 9N group(P＜0.05).The expression level of colonic MUC2 was increased significantly in 13.5N and 18N groups(P＜0.05)and decreased significantly in 22.5N group(P＜0.05).The expression levels of Claudin-2,Occludin,E-cadherin and ZO-1 in 18N and 22.5N groups were decreased significantly(P＜0.05).The abundance of Methanobrevibacter in 13.5N group,Streptococcus in 18N group and Lachnospira in 22.5N group was significantly increased(P＜0.05).L-tyrosine,L-glutamic acid,L-pyroglutamic acid,phenylalanine and other derivatives in 13.5N,18N group were significantly increased(P＜0.05),while significantly decreased(P＜0.05)in 22.5N group.Differential metabolites were mainly concentrated in amino acid metabolism pathway.In MS pigs and DLY pigs,there was a strong correlation between microflora and intestinal barrier related genes,as well as tryptophan and bile acid metabolites.[Conclusion]Improving the dietary fiber level could strengthen the intestinal barrier function of pigs,increase the abundance of beneficial bacteria,reduce the abundance of harmful bacteria,regulate the metabolism of lipid and amino acids in the colon,affect the metabolism of short-chain fatty acids,tryptophan and bile acids through the microbial pathway,and promote intestinal health.关键词
饲粮纤维水平/肠道屏障/微生物/代谢产物/猪Key words
dietary fiber level/intestinal barrier/microbiota/metabolites/pig引用本文复制引用
刘畅,赵燕,曹果清,崔子旭,左周,贠红梅,牛瑾,杨阳,郭晓红,李步高,高鹏飞..饲粮纤维水平对猪肠道屏障功能、结肠微生物及代谢产物的影响[J].中国农业科学,2023,56(22):4532-4551,20.基金项目
山西省高等学校科学研究优秀成果培育项目、山西省研究生创新项目(2021Y345)、山西农业大学生物育种工程项目(YZGC128)、三晋学者支持计划专项经费资助(2016 （2021Y345）
2017)、山西省现代农业产业技术体系建设专项 （）
