中国乳业Issue(11)：75-80,6.DOI:10.12377/1671-4393.23.11.14
酶联免疫法测定乳粉中黄曲霉毒素M1含量的不确定度评定
Uncertainty Assessment of Aflatoxin M1 ELISA Determination in Milk Powder
李婕 1曹学思 1杨爱君 1陈欣 1陈蓓蕾1
作者信息
- 1. 广东燕塘乳业股份有限公司,广东广州 510000
- 折叠
摘要
Abstract
[Objective]This article discussed the quantification and evaluation of the uncertainty of ELISA detection method for aflatoxin M1 residue in milk powder.[Method]By established a mathematical models,the factors of the introduction of uncertainty are analyzed by combining the fishbone diagram,and the uncertainty introduced by them was specifically quantified and compared.Thus,at a confidence level of 95%and containing factor k=2,the extended uncertainty of the determination of aflatoxin M1 in milk powder is:0.023 μg/kg.[Result]The M1 content of Aspergillus flavus in milk powder determined by ELISA method in this article should be expressed as:0.480±0.023 μg/kg.Among them,the factors that have a large influence on this uncertainty,the uncertainty introduced by standard curve fitting.[Conclusion]In the future inspection,the number of additional standard samples or quality control sample tests can be increased as appropriate to reduce the impact of the uncertainty introduced by this item on the inspection results.关键词
乳粉/黄曲霉毒素M1/不确定度/ELISAKey words
mink powder/AFM1/uncertainty/ELISA引用本文复制引用
李婕,曹学思,杨爱君,陈欣,陈蓓蕾..酶联免疫法测定乳粉中黄曲霉毒素M1含量的不确定度评定[J].中国乳业,2023,(11):75-80,6.
