中国输血杂志2023，Vol.36Issue(11)：978-986,9.DOI:10.13303/j.cjbt.issn.1004-549x.2023.11.002
基于培养组学和宏基因组学的血小板细菌检测方法研究
Detection of platelet bacteria based on cultomics and metagenomics
摘要
Abstract
Objective To explore the composition of culturable bacteria in platelets through bacterial culturomics and verify the results of culturomics and metagenomics to improve the detection rate of bacteria in platelets.Methods Platelet samples from 6 healthy people were collected.Eight kinds of culture media were placed in aerobic conditions and 12 kinds of culture media were placed in anaerobic conditions for large-scale culture and isolation of bacteria in platelets.The isolated single colony was identified by 16S rRNA gene sequencing.The bacterial abundance of healthy human platelet microbiome was analyzed by metagenomic sequencing,and the cultivable bacterial species in platelets was confirmed based on met-agenomic and culturomics results.Results A total of 90 strains of bacteria belonging to 3 phylums,5 classes,5 orders,7 families,9 genus and 23 species were isolated from 6 platelet samples by culturomics.Among them,the strains with more monoclonal clones at the species level were Brevundimonas aurantiaca(16.7%),Bacillus sp.Y1(15.6%),Cutibacterium acnes(14.4%)and Brevibacillus brevis(13.3%).The platelet samples sequenced by mNGS showed that the abundance val-ues of Proteobacteria,Firmicutes and Actinobacteria were high.The bacteria detected by both culturomics and metagenomic sequencing methods were as follows:Firmicutes:Bacillus sp.Y1,B.thuringiensis,B.cereus,B.mobilis,B.velezensis,Staph-ylococcus epidermidis,and Brevibacillus brevis;Actinobacteria:Cutibacterium acnes;Proteobacteria:Escherichia coli and Delft-ia tsuruhatensis.Conclusion The mutual validation of culturomics and metagenomics has identified some bacteria,proving that bacteria exist in platelets.关键词
血小板/宏基因组学/培养组学/细菌检测Key words
platelets/metagenomics/culturomics/bacteria detection分类
临床医学引用本文复制引用
赵梦伊,刘安庆,赵玉伟,戎霞,单振刚,高瞻,黄杨,何苗..基于培养组学和宏基因组学的血小板细菌检测方法研究[J].中国输血杂志,2023,36(11):978-986,9.基金项目
中国输血协会圣湘发展基金(CSBT-SX-2021-01) （CSBT-SX-2021-01）
2022年成都市医学科研课题(2022027) （2022027）
四川省国际科技创新合作(2021YFH0063) （2021YFH0063）
广州市科技计划(202002030192) （202002030192）
