中国输血杂志2023，Vol.36Issue(11)：1022-1025,4.DOI:10.13303/j.cjbt.issn.1004-549x.2023.11.012
人类嗜T淋巴细胞病毒抗体血清学筛查与确证试验的相关性分析
Correlation between serological screening of human T-lymphotropic virus antibodies and confirmatory tests
摘要
Abstract
Objective To explore the correlation between serological screening of human T-lymphotropic virus antibodies(anti HTLV)and Western blot(WB)confirmatory tests among blood donors,so as to explore the infection status of HTLVⅠ/Ⅱ in Guangzhou.Methods The anti HTLVⅠ/Ⅱenzyme-linked immunosorbent assay(ELISA)kit was used to screen voluntary blood donors from Guangzhou Blood Center from July 2016 to August 2022.WB was used to confirm 395 reactive blood samples by ELISA.The correlation between the S/CO values of anti HTLVⅠ/ⅡELISA reagents and the confirmatory test was analyzed using ROC curves.Results The results showed that 25 out of 395 initially screened reactive blood donor samples were confirmed as HTLV positive by WB,while 16 were uncertain.ROC curve analysis showed a correlation between the S/CO values by ELISA and the confirmatory test results:the S/CO value at the highest Youden index was 3.789,which was the optimal threshold.The S/CO value had a certain correlation with the predicted positive rate of confirmatory results(P＜0.05):the larger the S/CO value,the higher the predicted positive value.The overall prevalence of HTLV in Guangzhou is relatively low.Conclusion The prevalence of HTLV among blood donors in Guangzhou is low.Since the false positive rate of HTLV Ⅰ/Ⅱ antibody by ELISA serological screening is high,the confirmatory testing is particularly important.关键词
人类嗜T淋巴细胞病毒/S/CO值/ROC曲线/献血者Key words
human T-lymphotropic virus(HTLV)/S/CO value/ROC curve/blood donors分类
医药卫生引用本文复制引用
邓燕清,王淏,单振刚,谢君谋,杜荣松,肖巽南,李仲平,戎霞,黄伯泉..人类嗜T淋巴细胞病毒抗体血清学筛查与确证试验的相关性分析[J].中国输血杂志,2023,36(11):1022-1025,4.基金项目
广州市医学重点学科(2021-2023) （2021-2023）
广州市科技计划基金项目(2023A03J1000) （2023A03J1000）
