中国兽医科学2023，Vol.53Issue(12)：1480-1487,8.DOI:10.16656/j.issn.1673-4696.2023.0205
基于环介导等温扩增的三种马属动物疫病病原核酸检测方法的建立
A nucleic acid detection method for three equine pathogens based on loop-mediated isothermal amplification
摘要
Abstract
In this study,recombinant plasmids(named pUC57-AHSV,pUC57-WNV and pUC57-JEV)were con-structed to mimic the viruses according to the gene sequences of GenBank(GenBank accession No.:NC_006011,No.:NC_009942 and No.:NC_001437).Primers were synthesized and performed by loop-mediated isothermal amplification.The specificity,sensitivity,and reproducibility of this detection method were evaluat-ed.The results show that the method can detect the target specifically in one hour and the lowest detec-tion limit reaches 10 copies.The repeatability verification shows that the coefficient of variation of Cq value is less than 5％.In brief,a fast,low-cost,specific,and sensitive LAMP detection method was es-tablished,which can be used for effective monitoring of equines and rapid detection of pathogens.关键词
非洲马瘟病毒/西尼罗病毒/日本脑炎病毒/环介导等温扩增/核酸检测Key words
African horse sickness virus/West Nile virus/Japanese encephalitis virus/loop-medi-ated isothermal amplification/nucleic acid test分类
农业科技引用本文复制引用
高家乐,钮冰,陈沁,王艳..基于环介导等温扩增的三种马属动物疫病病原核酸检测方法的建立[J].中国兽医科学,2023,53(12):1480-1487,8.基金项目
上海市科技兴农项目(2020-02-08-00-10-F01470) （2020-02-08-00-10-F01470）
