中国兽医科学2023，Vol.53Issue(12)：1495-1506,12.DOI:10.16656/j.issn.1673-4696.2023.0215
FAdV-4感染鸡引起不同组织DNA识别受体及炎性因子表达的变化
Change of expression of DNA sensors and inflammatory cytokines in different tissues of chickens post FAdV-4 infection
摘要
Abstract
To examine the effect of the fowl adenovirus(FAdV-4)infection on avian innate immune response,the mRNA expressions of DNA sensors and inflammatory cytokines in different tissues from 21-day-old specific-pathogen-free(SPF)chickens with FAdV-4 infection,including heart,liver,spleen,lung,kidney,crop,thymus,trachea,esophagus,proventriculus,gizzard,pancreas,bursa of Fabricius,duodenum,jejunum,ileum,cecum,rectum,testis and brain,were examined by quantitative real-time PCR(qRT-PCR).Our data show that mRNA expressions of DNA sensors and down stream inflammatory cytokines were enhanced in most tissues in FAdV-4-infected chickens as compared to that of uninfected controls,especially in the heart and liver.The liver from chickens with FAdV-4 infection exhibited the highest level of viral replication as demonstrated by viral hexon mRNA expression in all tissues.These find-ings indicate that innate immune response to FAdV-4 infection differs in different tissues.These re-sults may provide reference for further research on the pathogenesis of FAdV-4 infection and the host innate immune response to FAdV-4.关键词
禽腺病毒血清4型/先天性免疫/DNA识别受体/炎性因子Key words
FAdV-4/innate immune/DNA sensor/inflammatory cytokine分类
农业科技引用本文复制引用
焦惠暄,郭雨欣,施佳蕾,王永强,高丽,曹红,李晓齐,郑世军..FAdV-4感染鸡引起不同组织DNA识别受体及炎性因子表达的变化[J].中国兽医科学,2023,53(12):1495-1506,12.基金项目
国家蛋鸡产业技术体系项目(CARS-40) （CARS-40）
北京市自然科学基金项目(6222037) （6222037）
