中国兽医科学2023，Vol.53Issue(12)：1516-1522,7.DOI:10.16656/j.issn.1673-4696.2023.0206
犬细小病毒颗粒疫苗的研制与免疫效果评价
Preparation and immunogenicity of virus-like particles of canine parvovirus
摘要
Abstract
Canine parvovirus enteritis is a highly infectious disease caused by canine parvovirus(canine parvovirus,CPV).At present,vaccine immunization remains the most effective means for disease control.In this study,the CPV VP2 gene was cloned into the pQB3 vector,the pQB3-VP2 recombinant plas-mid and qBac-ⅢG bacmid were co-transfected into sf9 insect cells to rescue the recombinant bac-ulovirus rBac-VP2,which can induce the cytopathic effect to sf9 insect cells and express CPV VP2 pro-tein with green fluorescent protein,the red fluorescence of the recombinant baculovirus rBac-VP2 was also observed by Indirect immunofluorescence assay.VP2 protein was identified clearly at about 65 ku band by Western-blot.The particles with similar morphology and size like parvovirus were observed un-der electron microscope,indicating that rBac-VP2 could assemble into virus-like particles(VLPs)after in-fecting sf9 insect cells,and the hemagglutination titer of VLPs was 1∶210.The CPV VLPs can induce the titer of 1∶27 hemagglutination inhibition antibody after immunizing mice,which was equivalent to the commercial vaccine of CPV.This study laid a foundation for the development of new genetic engineering vaccine of CPV.关键词
犬细小病毒/新型疫苗/病毒样颗粒/杆状病毒Key words
canine parvovirus/novel vaccines/virus-like particles/baculovirus分类
农业科技引用本文复制引用
李昀真,代昕宇,李双双,许丽文,张成琪,胡博,白雪..犬细小病毒颗粒疫苗的研制与免疫效果评价[J].中国兽医科学,2023,53(12):1516-1522,7.基金项目
国家自然科学基金项目(32100408) （32100408）
吉林省科技发展计划项目(20200402111NC,20210202050NC,20210509057RQ,20230202090NC) （20200402111NC,20210202050NC,20210509057RQ,20230202090NC）
