中国兽医科学2023，Vol.53Issue(12)：1537-1543,7.DOI:10.16656/j.issn.1673-4696.2023.0194
2,3,5-氯化三苯基四氮唑(TTC)在支原体培养中的应用研究
Application of 2,3,5-triphenyltetrazolium chloride(TTC)in Mycoplasma culture
摘要
Abstract
To analyze the application of 2,3,5-triphenyltetrazolium chloride(TTC)as a bacterial culture indicator in Mycoplasma culture.Mycoplasma bovis,Mycoplasma mycoides subsp.capri,Mycoplasma capricolum subsp.capripneumoniae,Mycoplasma ovipneumoniae,Mycoplasma gallisepticum and Mycoplasma pneumoniawere cultured in modified Thiaucourt's liquid culture medium(MTB)and modified Thiaucourt's solid culture medium(MTA)with TTC,and MTB and MTA added the phenol red indicator as culture control.The growth of Mycoplasma was observed in both TTC medium and phenol red medium,then the optimal concen-tration of TTC was screened.The results showed that TTC indicator exhibited more sensitive than con-ventional phenol red in the culture medium of Mycoplasma.In MTA medium with TTC as indicator,Mycoplas-mal colonies were more valuable for observing and counting colony-forming units(CFU).The optimal con-centration of TTC in Mycoplasma medium was 0.2 mg/mL.This study will provide basic on the optimization of Mycoplasma culture and vaccine production process.关键词
2,3,5-三苯基氯化四氮唑(TTC)/生长指示剂/支原体Key words
2,3,5-triphenyltetrazolium chloride(TTC)/indicator of growth/Mycoplasma分类
农业科技引用本文复制引用
刘立元,许健,张瑜瑜,宋银娟,支飞杰,储岳峰,秦建华..2,3,5-氯化三苯基四氮唑(TTC)在支原体培养中的应用研究[J].中国兽医科学,2023,53(12):1537-1543,7.基金项目
甘肃省科技重大专项(22ZD6NA001-3) （22ZD6NA001-3）
甘肃省重点人才项目(NKLS2020-119) （NKLS2020-119）
兰州兽医研究所揭榜挂帅项目(CAAS-ASTIP-JBGS-20210701) （CAAS-ASTIP-JBGS-20210701）
