广东海洋大学学报2023，Vol.43Issue(6)：1-8,8.DOI:10.3969/j.issn.1673-9159.2023.06.001
饲料DHA与EPA质量比对斜带石斑鱼稚鱼生长性能、体组成及消化酶活力的影响
Effects of Dietary DHA/EPA Mass Ratio on Growth Performance,Body Composition and Digestive Enzyme Activity of Larval Grouper(Epinephelus coioides)
摘要
Abstract
[Objective]The present study was conducted to investigate the effects of the mass ratio of dietary docosahexaenoic acid to eicosapentaenoic acid(DHA/EPA)on growth performance,body composition and digestive enzymes activities of grouper(Epinephelous coioides)larvae.[Method]Five isoproteic(58%)and isolipidic(16%)diets were formulated with graded DHA/EPA(0.82,1.28,1.67,2.00 and 2.33),named group 0.82,1.28,1.67,2.00 and 2.33,respectively,through adjusting the content of DHA enriched oil and EPA enriched oil.A 4-week feeding trial was conducted on larval grouper with an initial weight of(70±0.2)mg.Each group had 3 replicates and was fed to apparent satiation 5 times daily.[Results]The ratio of dietary DHA/EPA produced no significant difference on the survival rate of larval grouper(P>0.05),while the larvae in group 1.67 obtained significantly higher growth than other groups(P<0.05).The increase of dietary DHA/EPA significantly elevated crude lipid content of grouper larvae(P<0.05).Meanwhile,crude protein content of larvae in group 1.67 was significantly higher(P<0.05),while the moisture content in group 1.67 was significantly lower than other groups(P<0.05).The fatty acid composition of whole fish body and muscle was well correlated with dietary fatty acid profile,and DHA was easier to be retained compared to EPA(P<0.05).The activity of digestive enzymes was significantly affected by the ratio of dietary DHA/EPA(P<0.05).The activity of pepsin and intestinal trypsin in grouper larvae in group 1.67 was statistically higher than that of other groups(P<0.05).However,the increase of the ratio of dietary DHA/EPA significantly reduced the activity of intestinal amylase,and the intestinal lipase activity in groups 0.82 and 2.33 was significantly lower than that of other groups(P<0.05).[Conclusion]Optimal ratio of dietary DHA/EPA significantly improved the growth performance,and promoted the development of digestive tract of larval grouper,and dietary DHA/EPA ratio should be maintained at 1.67 for the micro-diets for this grouper.关键词
斜带石斑鱼/微颗粒饲料/DHA/EPA/生长性能/消化酶活力Key words
Epinephelous coioides/micro-diet/DHA/EPA/growth performance/digestive enzyme activity分类
农业科技引用本文复制引用
袁禹惠,龚埜,黄岩,李松林..饲料DHA与EPA质量比对斜带石斑鱼稚鱼生长性能、体组成及消化酶活力的影响[J].广东海洋大学学报,2023,43(6):1-8,8.基金项目
科技部蓝色粮仓科技创新重大专项基金(2019YFD900200) （2019YFD900200）
