广东海洋大学学报2023，Vol.43Issue(6)：9-18,10.DOI:10.3969/j.issn.1673-9159.2023.06.002
饲料磷脂水平对乌鳢幼鱼生长性能、抗氧化能力及脂肪沉积的影响
Effects of Dietary Phospholipid Level on Growth Performance,Antioxidant Capacity and Fat Deposition of Juvenile Snakehead(Channa argus)
摘要
Abstract
[Objective]The aim of this paper is to explore the effects of dietary phospholipid level on growth performance,antioxidant capacity,fatty acid composition and lipid metabolism of juvenile snakehead(Channa argus).[Methods]Snakehead fry with an initial body mass of(3.99±0.01)g were fed with five iso-nitrogenous and iso-lipidic diets prepared by adding 0,2%,4%,6%and 8%phospholipids to the basal diet,respectively designated as group PL0(control group),PL2,PL4,PL6 and PL8.At the end of the 43-day's culture experiment,the growth indicators,physiological and biochemical indicators and the expression levels of lipid metabolism-related genes in the liver of the juvenile snakehead were measured.[Results]1)The addition of phospholipids in the diet significantly affected the growth performance of juvenile snakehead.The final body mass and specific growth rate of the juvenile snakehead in group PL4 and group PL6 were significantly higher than those in group PL0(P<0.05).The daily feed intake(FI)and feed conversion ratio(FCR)showed a trend of decreasing first and then increasing.The FI in group PL4 was the lowest,and the FCR in group PL6 was the lowest,both of which were significantly lower than those in group PL0(P<0.05).Meanwhile,the viscerosomatic index(VSI)of the fry in group PL8 was significantly lower than that of the other groups(P<0.05).2)With the increase of dietary phospholipid level,the increase of phospholipid content in the diet significantly reduced the crude fat content of the whole fish and liver,while the moisture showed the opposite trend(P<0.05).3)With the increase of dietary phospholipid level,the malondialdehyde content in the liver decreased more significantly in groups PL6 and PL8 than in the other groups(P<0.05);the superoxide dismutase activity in the liver increased more significantly in group PL8 than in the other groups(P<0.05);the total antioxidant capacity increased first and then decreased,and the capacity in group PL2 was significantly higher than that of the other groups except PL6(P<0.05);the catalase activity was not affected by the change of phospholipid content in the diet(P>0.05).4)The addition of phospholipids in the diet significantly increased the n-3PUFA content of the fish body and liver(P<0.05).5)The addition of phospholipids also had a significant effect on the expression of lipid metabolism-related genes in the liver.The expression level of acc1,a lipid synthesis-related gene,in group PL8 was significantly lower than that in PL0 group(P<0.05);the expression levels of mgl and hsl,lipid degradation-related genes,in groups PL4 and PL6 were significantly higher than those in group PL0(P<0.05),and hsl in group PL6 was significantly higher than that in group PL0(P<0.05);the expression levels of pparα,cpt1 and aco,fatty acid oxidation-related genes,in group PL6 were significantly higher than those in group PL0(P<0.05).[Conclusion]Adding phospholipids to the diet can significantly improve the growth performance of juvenile snakehead,and enhance their antioxidant capacity and reduce lipid deposition by regulating lipid metabolism.The suitable dietary phospholipid requirement for juvenile snakehead was 5.36%.关键词
乌鳢/磷脂/生长性能/抗氧化能力/脂质代谢Key words
Channa argus/phospholipids/growth performance/antioxidant capacity/lipid metabolism分类
农业科技引用本文复制引用
张玉,龚埜,李文飞,王振杰,陈乃松,李松林..饲料磷脂水平对乌鳢幼鱼生长性能、抗氧化能力及脂肪沉积的影响[J].广东海洋大学学报,2023,43(6):9-18,10.基金项目
国家现代农业产业技术体系项目(CARS-46) （CARS-46）
