广东海洋大学学报2023，Vol.43Issue(6)：19-27,9.DOI:10.3969/j.issn.1673-9159.2023.06.003
胍基乙酸对许氏平鲉生长性能、消化吸收、代谢及抗氧化能力的影响
Effects of Guanidinoacetic Acetic Acid on Growth Performance,Digestion and Absorption,Metabolism and Antioxidant Capacity of Sebastes schlegelii
摘要
Abstract
[Objective]To investigate the effects of guanidinoacetic acid(GAA)on growth performance,digestion and absorption,metabolism and antioxidant capacityantioxidant capacity of Sebastes schlegelii and to provide theoretical basis for the application of GAA in the cage culture of S.schlegelii.[Method]Five iso-nitrogenous and iso-lipid experimental diets were prepared by adding GAA with mass fraction of 0.00%(D0),0.01%(D1),0.02%(D2),0.04%(D3),0.08%(D4)respectively to the basal diet and fed to five groups of experimental rockfish[initial mass(125.00±1.00)g]for seven weeks.Growth performance,antioxidant,serum biochemical indices and liver glycometabolism of S.schlegelii were determined.[Result]The results in the current study indicated that:(1)Compared with control group(D0),groups D1,D2,D3 and D4 significantly increased the body weight gain rate,specific growth rate and protein efficiency,and decreased the feed coefficient(P<0.05).(2)There were no significant differences in moisture,crude protein,crude lipid and ash content of whole fish among the five groups(P>0.05).(3)Compared with group D0,the activities of intestinal trypsin and amylase in groups D1,D2,D3 and D4 were significantly increased(P<0.05),while the activities of lipase in groups D3 and D4 were significantly increased(P<0.05).(4)Compared with group D0,serum triglyceride and high density lipoprotein cholesterol concentrations in group D3 were significantly increased(P<0.05).(5)Compared with group D0,T-AOC content in liver in groups D1,D2,D3 and D4 was significantly increased(P<0.05),malondialdehyde content was significantly decreased in groups D1,D2,D3 and D4(P<0.05),and SOD activity in groups D2,D3 and D4 was significantly increased(P<0.05).(6)Compared with group D0,the PK activity and CK activity in liver of groups D1,D2,D3 and D4 were significantly decreased(P<0.05),the PFK activity was significantly decreased in group D3(P<0.05),and the Cr content in liver of groups D2,D3 and D4 was significantly increased(P<0.05).[Conclusion]With the weight gain rate as the evaluation indictor,the appropriate supplemental level of GAA in the diet of S.schlegelii is 0.04%.The addition of suitable GAA(0.04%-0.08%)in the diet can improve the intestinal digestive enzyme activity of S.schlegelii,and regulate the content of metabolites to exert antioxidant effect and energy-regulating effect.关键词
胍基乙酸/许氏平鲉/生长性能/消化吸收/代谢/抗氧化能力Key words
guanidinoacetic acid/Sebastes schlegelii/growth performance/digestion and absorption/metabolism/antioxidant capacity分类
农业科技引用本文复制引用
郝甜甜,于朝磊,孙永智,李宝山,王晓艳,王成强,李璐,黄炳山..胍基乙酸对许氏平鲉生长性能、消化吸收、代谢及抗氧化能力的影响[J].广东海洋大学学报,2023,43(6):19-27,9.基金项目
山东省重点研发计划(科技示范工程)(2021SFGC0701) （科技示范工程）
山东省自然科学基金青年基金(ZR2020QC206) （ZR2020QC206）
