广东海洋大学学报2023，Vol.43Issue(6)：28-36,9.DOI:10.3969/j.issn.1673-9159.2023.06.004
包膜丁酸钠对大规格刺参生长性能、消化、抗氧化和相关基因表达的影响
Effects of Coated Sodium Butyrate on Growth Performance,Digestive Capacity,Antioxidant Capacity and Related-gene mRNA Expression of Large-size Sea Cucumber(Apostichopus japonicus)
摘要
Abstract
[Objective]This study aimed to evaluate the effects of coated sodium butyrate in diets on growth performance,digestive capacity,antioxidant capacity and related-gene mRNA expression of large-size sea cucumber(Apostichopus japonicus),and provided theoretical basis for the rational utilization of sodium butyrate in culturing sea cucumber.[Method]Six isonitrogenous and isoenergetic diets were formulated with graded mass fraction of dietary coated sodium butyrate,0,0.05%,0.10%,0.20%,0.40%and 0.60%respectively,and the non-added group was the control group.The feeding experiment lasted for 8-week,and the initial mass of sea cucumber was(55.96±0.20)g.[Result]The results showed that no significant differences in survival rate were found among dietary treatments(P>0.05).With the increase of dietary sodium butyrate levels,the weight gain rate(WGR)and specific growth rate(SGR)increased,and then decreased after reaching the peak at the 0.40%addition group,significantly higher than those of control,0.05%,0.10%addition groups(P<0.05).The body wall of Apostichopus japonicus composition analysis showed that the crude protein,crude lipid,crude ash and moisture content of body wall were not significantly different in different groups(P>0.05).The activities of protease and lipase in intestinal tract reached their peaks at the 0.40%addition group,with no significant differences from those of 0.60%addition group(P>0.05),while significantly higher than those of control group(P<0.05).On the antioxidant capacity of sea cucumber,the activities of SOD,CAT and T-AOC in intestinal tract of 0.20%,0.40%and 0.60%addition groups were significantly higher than those of control group(P<0.05).But the MDA content in intestinal tract changed in a trend opposite to that of SOD(P<0.05).The relative expression of Rel in intestinal tract of Apostichopus japonicus was the highest in 0.40%addition group,which was significantly higher than that in the control group(P<0.05).At the same time,the relative expression of P105 in intestinal tract changed in a trend opposite to that of Rel.The relative expression of lysozyme in intestinal tract was the highest in 0.40%addition group,which was significantly higher than that in control group and 0.60%addition group(P<0.05).[Conclusion]The results showed that 0.40%coated sodium butyrate could promote the growth performance,digestibility and antioxidant capacity of large-size Apostichopus japonicus.In addition,sodium butyrate can affect the anti-inflammatory level of Apostichopus japonicus by regulating the related factors of NF-κB signal pathway,and then have a positive effect on the immune ability of the body.关键词
刺参/包膜丁酸钠/生长性能/抗氧化能力/基因表达Key words
Apostichopus japonicus/coated sodium butyrate/growth performance/antioxidant capacity/gene expression分类
农业科技引用本文复制引用
王成强,李宝山,孙永智,王晓艳,郝甜甜,相智巍,刘财礼,王佩锋,王际英..包膜丁酸钠对大规格刺参生长性能、消化、抗氧化和相关基因表达的影响[J].广东海洋大学学报,2023,43(6):28-36,9.基金项目
山东省现代农业产业技术体系刺参产业技术体系(SDAIT-22-06) （SDAIT-22-06）
海洋生物资源的开发与利用项目(220-0110-JBN-54EY) （220-0110-JBN-54EY）
