广东海洋大学学报2023，Vol.43Issue(6)：72-80,9.DOI:10.3969/j.issn.1673-9159.2023.06.009
不同体质量黄鳍金枪鱼能量代谢相关酶活性与基因表达
Enzyme Activity and Gene Expression Related to Energy Metabolism of Yellowfin Tuna with Different Body Mass
摘要
Abstract
[Objective]The aim of this study is to investigate the differences in energy metabolism between red and white muscle tissues of yellowfin tuna(Thunnus albacares)of different body mass.[Methods]The glycogen content,anaerobic and aerobic metabolic key enzyme activity and metabolism-related gene expression were measured in red and white muscle of small fish(0.85±0.03)kg,middle fish(9.77±0.15)kg and large fish(19.63±0.37)kg were measured.[Results]1)Glycogen content in red muscle was the highest in large fish group and the lowest in small fish group(P<0.05).And the content of glycogen in white muscle was the highest in middle fish,and glycogen content in red muscle of different body mass groups were extremely significantly higher than that in white muscle(P<0.01).2)The activity of anaerobic metabolism key enzymes,including hexokinase(HK),pyruvate kinase(PK),lactate dehydrogenase(LDH)and phosphofructokinase(PFK)in red and white muscle of large fish group were significantly higher than those in small and middle fish(P<0.05),and the activities of HK,PK,LDH and PFK in white muscle of large fish group were significantly higher than those in red muscle(P<0.05);The activity of aerobic metabolism key enzymes,including citrate synthase(CS),succinate dehydrogenase(SDH)and malate dehydrogenase(MDH),were much higher in large fish group than in the other two groups(P<0.05).And CS and SDH activities in red muscle were significantly higher than those in white muscle tissue in large fish group(P<0.05).3)There were significant differences in the expression of the hk,sdh and mdh genes in the red muscle,with the highest expression in the large fish group(P<0.05),the ldh and pk gene expressions had no correlation with body mass,the expression of cs and pfk gene were higher than other two groups(P<0.05);The expression levels of sdh and mdh genes in white muscle with different body mass were significantly different(P<0.05),the hk,pk and cs gene expressions in middle fish group were significantly higher than those in other groups(P<0.05),but pfk gene expression was not significantly different,and the ldh gene expression was the highest in large fish group.The expressions of cs and sdh genes in red muscle were significantly higher than those in white muscle in the large fish group(P<0.01),and the expressions of hk,pk and ldh genes in white muscle were extremely higher than those in red muscle in the middle and large groups(P<0.01).4)Body mass was the main factor affecting glycogen content and enzyme activity.[Conclusion]Tuna muscle glycogen content,anaerobic and aerobic metabolic enzyme activity and gene expression are influenced by changes in fish body mass.关键词
黄鳍金枪鱼/体质量/能量代谢/基因表达/红肌/白肌Key words
Thunnus albacares/body mass/energy metabolism/gene expression/red muscle/white muscle分类
农业科技引用本文复制引用
李倩,李钰,符文雅,肖娟,黄海,郭志强..不同体质量黄鳍金枪鱼能量代谢相关酶活性与基因表达[J].广东海洋大学学报,2023,43(6):72-80,9.基金项目
海南热带海洋学院/海南热带海洋学院崖州湾创新研究院开放课题(2022RHDKFKT03) （2022RHDKFKT03）
