中南大学学报（自然科学版）2023，Vol.54Issue(11)：4238-4249,12.DOI:10.11817/j.issn.1672-7207.2023.11.003
Klebsiella aerogenes的胞外聚合物产量优化及其对碳酸钙晶型的调控作用
Optimization of EPS production and its regulation for calcium carbonate crystals form by Klebsiella aerogenes
摘要
Abstract
The optimal culture conditions of extracellular polymeric substances(EPS)produced by Klebsiella aerogenes were determined by response surface,and the experiments were conducted to investigate the effect of EPS on the formation of calcium carbonate crystals induced by Klebsiella aerogenes.The results show that the effects of cultivation time and temperature on EPS production are more significant than those of pH and inoculation amount by Klebsiella aerogenes.The optimal cultivation conditions for EPS production are the cultivation time of 4.64 d,cultivation temperature of 28.35℃,inoculation amount of 2.97%,and cultivation pH of 7.69.Under the optimal cultivation condition,the EPS production reaches 210.24 mg/L.The percentage of calcite increase from 27.1%to 98.3%and the percentage of vaterite decreased from 72.9%to 1.7%,when the additional EPS mass concentration is increased from 0 g/L to 1.0 g/L.EPS can effectively promote the formation of calcite and inhibit the formation of vaterite.The negatively charged functional groups in EPS can adsorb Ca2+ from the solution,resulting in the reduction of Ca2+ concentration and CaCO3 supersaturation in the solution,which promotes the formation of calcite.The ratio of calcite and vaterite induced by Klebsiella aerogenes can be regulated by additional EPS.The conversion of calcium carbonate crystal to calcite with thermodynamic stability is enhanced by the EPS of Klebsiella aerogene,which provides a new way for improving the mechanical strength of solidified samples induced by microbial induced calcium carbonate precipitation.关键词
Klebsiella aerogenes/胞外聚合物/微生物诱导碳酸钙沉淀/晶型调控Key words
Klebsiella aerogenes/extracellular polymeric substances/microbial induced calcium carbonate precipitation/crystal form regulation分类
天文与地球科学引用本文复制引用
胡南,姚顺超,李艾书,朱家华,段重达,李广悦,杨志辉,丁德馨..Klebsiella aerogenes的胞外聚合物产量优化及其对碳酸钙晶型的调控作用[J].中南大学学报（自然科学版）,2023,54(11):4238-4249,12.基金项目
国家自然科学基金资助项目(U20A20267,12275123,U1967210) （U20A20267,12275123,U1967210）
湖南省杰出青年基金资助项目(2022JJ10041) （2022JJ10041）
湖南省科技人才托举项目(2020TJ-Q03) （2020TJ-Q03）
湖南省重点研发计划项目(2022SK2077)(Projects(U20A20267,12275123,U1967210)supported by the National Natural Science Foundation of China （2022SK2077）
Project(2022JJ10041)supported by the Hunan Provincial Science Fund for Distinguished Young Scholars （2022JJ10041）
Project(2020TJ-Q03)supported by the Science and Technology Talent Developing Foundation of Hunan Province （2020TJ-Q03）
Project(2022SK2077)supported by the Key Research and Development Program of Hunan Province) （2022SK2077）
