作物学报2024，Vol.50Issue(1)：16-31,16.DOI:10.3724/SP.J.1006.2024.31013
小麦骨干亲本周8425B抗条锈病优异基因在其衍生品种中的遗传解析
Genetic analysis of elite stripe rust resistance genes of founder parent Zhou 8425B in its derived varieties
摘要
Abstract
Zhou 8425B is a widely used dwarf large panicle and disease-resistant and stress-resistant Wheat Founder Parent in the wheat production areas of the Yellow-Huaihe-Haihe Rivers regions.The analysis of stripe rust resistance of derived varieties and the genetic transmission information of stripe rust resistance gene carried by Zhou 8425B is of great value for wheat new variety breeding.In this study,we used a highly toxic race of stripe rust,Tiaozhong 34(CYR34),to identify the resistance of 222 col-lected Zhou 8425B derived varieties to stripe rust at seedling stage.The hybrid strain mainly CYR34 was used to identify the re-sistance to stripe rust at adult stage of the derived varieties.Subsequently,molecular markers closely linked to the stripe rust re-sistance genes(YrZH84,YrZH84.2,Yr30,YrZH22,and Yr9 carried by Zhou 8425B)were used for genotype detection of the de-rived varieties.The results showed that Zhou 8425B was highly resistant to stripe rust stripe rust at seedling stage and adult stage to the current virulent dominant race CYR34.Among the 222 derivative varieties of Zhou 8425B,14 of them,including Changmai 9,Jiyanmai 10,Bainong 4199,Saidemai 7,and Zhengmai 103,etc.,showed stable disease resistance in two years,accounting for 6.3%;52 derivative varieties,include Zhoumai 11,Zhoumai 22,Zhoumai 26,Zhoumai 36,Lantian 36,Cunmai 16 and Zheng-pinmai 8,etc.,had stable disease resistance during the whole growth period,accounting for 23.4%.Many derivative varieties of Zhou 8425B were mainly derived from six offspring,including Zhoumai 11,Zhoumai 12,Zhoumai 13,Zhoumai 15,Zhoumai 16,and Zhoumai 17.In the first generation,Zhoumai 16 and Zhoumai 13 directly derived more varieties because of their good agro-nomic characters,while Zhoumai 15 and Zhoumai 17 derived fewer varieties.Zhoumai 12 and Zhoumai 13 bred the second gen-eration,Zhoumai 22,and then derived 45 sub-generations,and Zhoumai 11 bred Aikang 58,and then derived 54 sub-generations.The excellent stripe rust resistance gene of Zhou 8425B was continuously separated and polymerized in the process of genetic breeding.The frequencies of YrZH84,YrZH84.2,YrZH22,Yr30,and Yr9 in the derived offspring were 34.7%,14.9%,41.9%,66.2%,and 67.1%,respectively.Among the derivative varieties carrying only one disease resistance gene,the average severity of YrZH84 was the lowest(14.4%).Among the derived varieties that aggregate two disease resistance genes,the average severity of carrying YrZH84+YrZH22 was the lowest(20.0%);among the derived varieties that aggregate three disease resistance genes,the average severity of carrying YrZH84+YrZH22+Yr9 was the lowest(17.2%).Among the derived varieties that aggregate four dis-ease resistance genes,the average severity of carrying YrZH84+YrZH22+Yr30+Yr9 was 16.9%,and the average severity of carry-ing YrZH84.2+YrZH22+Yr30+Yr9 was 38.4%.At seedling stage,derived varieties that carried the YrZH84 resistance gene or a combination of genes containing YrZH84 during the entire growth period had better disease resistance.These results provide the information of stripe rust gene for the continuous improvement and utilization of the Founder Parent Zhou 8425B in China,iden-tify the new derived germplasm with high resistance and high yield to the highly virulent physiological race CYR34,which pro-viding a reference for the genetic breeding of wheat resistance to stripe rust in China.关键词
小麦条锈病/周8425B/抗条锈病基因/遗传解析/衍生系Key words
wheat stripe rust/Zhou 8425B/stripe rust resistance gene/genetic analysis/derived varieties引用本文复制引用
李俣佳,郑天存,殷贵鸿,许豪,于士男,唐建卫,李巧云,高艳,郑继周,董纯豪,袁雨豪..小麦骨干亲本周8425B抗条锈病优异基因在其衍生品种中的遗传解析[J].作物学报,2024,50(1):16-31,16.基金项目
本研究由2022年度河南省重大科技专项(221100110700),河南省农业良种联合攻关项目(2022010102),河南省现代农业产业技术体系建设(HARS-22-01-G2),河南省科技研发计划联合基金(222103810002)和河南农业大学拔尖人才项目(30500678)资助. This study was supported by the Henan Province Major Science and Technology Special Project in 2022(221100110700),the Henan Province Agricultural Variety Joint Research Project(2022010102),the Henan Province Modern Agriculture Industry Technology System Construction(HARS-22-01-G2),the Henan Province Science and Technology Research and Development Plan Joint Fund(222103810002),and the Henan Agricultural University Top Talent Project(30500678). （221100110700）
