作物学报2024，Vol.50Issue(1)：55-66,12.DOI:10.3724/SP.J.1006.2024.33011
控制玉米株高基因PHR1的基因克隆
Mapping and cloning of plant height gene PHR1 in maize
摘要
Abstract
Plant height is an important index for the ideal maize breeding,which not only affects the mechanical harvest of maize,but also closely relates to the lodging resistance and the biological yield of maize.In this study,a plant height reducing mutant-1(phr-1)was obtained by using low-dose fast neutron(4.19 Gy)irradiation to mutate the maize inbred line KWS39 at the low ear position.Phenotypic traits were investigated and analyzed in the field,and candidate genes were identified by mining and func-tional annotation of genes in the target region based on the B73 reference genome using the extreme trait pool sequencing analysis(BSA-seq)and the method of target region recombination exchange identification with F2 segregation population of phr-1×B73.These results indicated that there might be a variation site in Bin1.06 interval on chromosome 1,and the target region was pre-cisely located between two markers,Umc1122 and Umc1583a,with a 600 kb interval by using the large segregation population and polymorphic markers.Brachytic2(BR2),encoding a sugar protein regulating the polar transport of auxin in maize stems,is a known gene controlling plant height in this region.The sequencing of candidate genes revealed that phr-1 was a new allelic muta-tion of br2-1,with a 165 bp insertion in the fourth exon of the BR2 gene resulting in the amino acid at position 547 changing to a stop codon and premature protein translation termination.The mutation site and variation mode of phr-1 were completely different from those of br2-1 single base mutation site.The allelic hybridization experiment confirmed that the PHR1 candidate gene is a new allelic mutation of the BR2 gene.This study provides new germplasm resources for maize BR2 gene in plant height genetic improvement.关键词
玉米/株高/穗位高/BSA混池测序/基因定位/功能分析Key words
maize/plant height/ear height/the bulked segregant analysis/map-based localization/function analysis引用本文复制引用
杨晨曦,连晓荣,李永生,周文期,周香艳,刘忠祥,周玉乾,刘芥杉,杨彦忠,何海军,王晓娟..控制玉米株高基因PHR1的基因克隆[J].作物学报,2024,50(1):55-66,12.基金项目
本研究由国家自然科学基金项目(32160490,31960443),甘肃省重大专项(21ZD11NA005,21ZD10NF003),甘肃省农业科学院生物育种专项(2022GAAS04),甘肃农业大学青年导师基金项目(GSAU-QDFC-2021-14)和中国工程院战略研究与咨询项目:特色粮油作物优异基因精准鉴定及新种质创制(2021-DFZD-21-3)资助. This study was supported by the National Natural Science Foundation of China(32160490,31960443),the Major Project of Gansu Province(21ZD11NA005,21ZD10NF003),the Biological Breeding Project of Gansu Academy of Agricultural Sciences(2022GAAS04),the Youth Mentor Fund Project of Gansu Agricultural University(GSAU-QDFC-2021-14),and the Strategic Research and Consulting Project of Chi-nese Academy of Engineering(2021-DFZD-21-3). （32160490,31960443）
