转基因抗虫耐除草剂玉米自交系LG11的获得及抗性分析OA

Insect pests are an important limiting factor affecting the yield and quality of maize.Bacillus thuringiensis transgenic maize has good insect resistance and can reduce the use of chemical insecticides effectively,which is very popular among growers.However,a large area of continuous planting of transgenic corn will lead to the resistance of target pests."Multi-gene"strategy is one of the management strategies to prevent or delay the occurrence of resistant populations of target pests.The anti-insect vector constructed in this study is a fusion protein M2cryAb-vip3Aa formed by combining the main structural domains of Cry1Ab and Vip3Aa proteins using synthetic methods.These two insecticidal proteins had no evolutionary homology and different insecticidal mechanisms,which could effectively reduce the resistance probability of target pests.In this study,to obtain a new transgenic maize material with insect resistance and herbicide resistance and excellent agronomic traits,and analyze its insect resistance and herbicide resistance,m2cryAb-vip3Aa and bar genes were introduced into the recipient materials in series by transgenic method,and Chang 7-2,a backbone inbred line,was used as the backcross parent for backcross transfer.The results of this study enriches the existing germplasm resources of insect-resistant and herbicide-tolerant maize,and provides new solutions for pest control and weed control in maize fields.