作物学报2024，Vol.50Issue(1)：89-99,11.DOI:10.3724/SP.J.1006.2024.33016
转基因抗虫耐除草剂玉米自交系LG11的获得及抗性分析
Acquisition and resistance analysis of transgenic Maize Inbred Line LG11 with insect and herbicide resistance
摘要
Abstract
Insect pests are an important limiting factor affecting the yield and quality of maize.Bacillus thuringiensis transgenic maize has good insect resistance and can reduce the use of chemical insecticides effectively,which is very popular among growers.However,a large area of continuous planting of transgenic corn will lead to the resistance of target pests."Multi-gene"strategy is one of the management strategies to prevent or delay the occurrence of resistant populations of target pests.The anti-insect vector constructed in this study is a fusion protein M2cryAb-vip3Aa formed by combining the main structural domains of Cry1Ab and Vip3Aa proteins using synthetic methods.These two insecticidal proteins had no evolutionary homology and different insecticidal mechanisms,which could effectively reduce the resistance probability of target pests.In this study,to obtain a new transgenic maize material with insect resistance and herbicide resistance and excellent agronomic traits,and analyze its insect resistance and herbicide resistance,m2cryAb-vip3Aa and bar genes were introduced into the recipient materials in series by transgenic method,and Chang 7-2,a backbone inbred line,was used as the backcross parent for backcross transfer.The results of this study enriches the existing germplasm resources of insect-resistant and herbicide-tolerant maize,and provides new solutions for pest control and weed control in maize fields.关键词
m2cryAb-vip3Aa/bar/转基因/玉米Key words
m2cryAb-vip3Aa/bar/transgenic/maize(Zea mays L.)引用本文复制引用
岳润清,李文兰,孟昭东..转基因抗虫耐除草剂玉米自交系LG11的获得及抗性分析[J].作物学报,2024,50(1):89-99,11.基金项目
本研究由泰山学者工程项目和财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-02-09)资助. This study was supported by the Taishan Scholars Program and China Agriculture Research System of MOF and MARA(CARS-02-09). （CARS-02-09）
