作物学报2024，Vol.50Issue(1)：126-137,12.DOI:10.3724/SP.J.1006.2024.34045
棉花AP2/ERF转录因子GhTINY2负调控植株抗盐性的功能分析
Function analysis of an AP2/ERF transcription factor GhTINY2 in cotton nega-tively regulating salt tolerance
摘要
Abstract
Cotton is a relatively salt-tolerant crop,but high salt stress leads to a significant decline in cotton yield and fiber quality.Mining the genes involved in salt-tolerance and illuminating the molecular mechanisms that underlie this resistance is of great importance in cotton breeding programs.Here,we identified an AP2/ERF transcription factor GhTINY2 in the transcriptome da-tabase from cotton treated with salt,and the relative expression level of GhTINY2 was reduced by salt.Subsequently,the salt-resistant phenotype and physiological indicators of the GhTINY2-overexpression Arabidopsis were analyzed.The results revealed that the GhTINY2-overexpression Arabidopsis had a significant decrease in seed germination rate,the content of proline,soluble sugar,and chlorophyll under salt stress,leading to more severe leaf wilting compared with WT.RNA-seq data from GhTINY2-transgenic Arabidopsis revealed that differentially expressed genes(DEGs)were enriched in a series of bio-logical processes,including chlorophyll metabolism and response to stimulus,and the relative expression level of these DEGs significantly was down-regulated.Moreover,the silence of GhTINY2 in cotton through Virus-induced gene silencing(VIGS)assay showed that TRV:GhTINY2 had a significant increase in chlorophyll and proline content,leading to improved salt tolerance com-pare with TRV:00.In conclusion,these findings suggest that GhTINY2 was an important gene in cotton that negatively regulated salt stress resistance,and it was expected to create salt-tolerant cotton materials using GhTINY2 gene by modern genetic engi-neering technology in the future.关键词
棉花/GhTINY2/盐胁迫/转录因子/转基因Key words
cotton/GhTINY2/salt stress/transcription factor/transgenic引用本文复制引用
肖胜华,陆妍,李安子,覃耀斌,廖铭静,闭兆福,卓柑锋,朱永红,朱龙付..棉花AP2/ERF转录因子GhTINY2负调控植株抗盐性的功能分析[J].作物学报,2024,50(1):126-137,12.基金项目
本研究由广西大学高层次人才科研启动基金项目(A3310051044)和广西大学农学院科研发展金项目(EE101711)资助. This study was supported by the Initial Scientific Research Fund of High-level Personnel in Guangxi University(A3310051044)and the Guangxi University Agricultural College Research Development Fund(EE101711). （A3310051044）
