作物学报2024，Vol.50Issue(1)：187-198,12.DOI:10.3724/SP.J.1006.2024.34046
低温下环丙酸酰胺调控棉花内源激素促进噻苯隆脱叶的机制
Mechanism of cyclanilide enhanced the defoliation efficiency of thidiazuron in cotton by regulating endogenous hormones under low temperature stress
摘要
Abstract
The technology of cotton leaf removal is an important prerequisite for realizing the mechanical harvesting of cotton,but low temperature affect the efficiency of TDZ-induced leaf abscission,which cannot meet the requirements of mechanized har-vesting.In our laboratory,we found that cyclanilide(CYC)could improve the defoliation efficiency of TDZ at low temperature,but the mechanism of CYC promoting chemical defoliation(TDZ)under low temperature is unknown.Therefore,the content of plant hormones and the relative expression level of related genes in the abscission zone of cotton leaves were analyzed using Zhongmian 425 as the experimental material,setting two temperature levels(25℃and 15℃)and three treatments[water(CK),single TDZ(T),and the compound of TDZ+CYC(TC)].At 240 h after treatment under low temperature(15℃),the abscission rate of cotton leaves treated by T was only 53.0%,while the start time of cotton leaves abscission treated by TC was 24 h ahead of that treated by T and the abscission rate of cotton leaves treated by TC increased to 79.6%.At low temperature,compared with T,the relative expression level of auxin(IAA)transport genes(LAX2,PIN1),IAA response genes(IAA9,ARF3)and the content of IAA in the abscission zone of cotton leaves treated with TC were reduced significantly;the relative expression level of ethylene(ET)synthesis genes(ACS,ACO),and the content of ET synthesis precursor ACC were increased,the relative expression level of ET downstream signal gene(ERF1B)was up-regulated significantly in the abscission zone treated with TC.The jasmonate acid(JA)synthesis-related gene(AOC4)was up-regulated,and the content of JA was increased in abscission zone treated by TC.The inhibition of the IAA transport and signal transduction and the promotion of ET and JA synthesis and ET signal transduction caused by TC treatment in the abscission zones of cotton leaves could be the key reason that CYC could promote cotton leaves abscission effect of TDZ at low temperature.关键词
棉花脱叶/低温/噻苯隆/环丙酸酰胺/内源激素含量/基因表达Key words
cotton defoliation/low temperature/thiadiazuron/cyclanilide/plant hormone content/gene relative expression引用本文复制引用
孙尚文,束红梅,杨长琴,张国伟,王晓婧,孟亚利,王友华,刘瑞显..低温下环丙酸酰胺调控棉花内源激素促进噻苯隆脱叶的机制[J].作物学报,2024,50(1):187-198,12.基金项目
本研究由国家自然科学基金项目(32071968),江苏省农业科技自主创新资金项目(CX(22)2015)和江苏省现代作物协同创新中心项目(JCIC-MCP)资助. This study was supported by the National Natural Science Foundation of China(32071968),the Jiangsu Agricultural Science and Technology Innovation Fund(CX(22)2015),and the Jiangsu Collaborative Center for Modern Crop Production(JCIC-MCP). （32071968）
