作物学报2024，Vol.50Issue(1)：219-236,18.DOI:10.3724/SP.J.1006.2024.34035
盐碱胁迫对棉花叶片蛋白质组的影响及差异性分析
Effects and variability analysis of different salt and alkali stresses on the proteome of cotton leaves
摘要
Abstract
Salt stress and alkali stress are two different abiotic stresses that seriously affect agricultural production.Exploring the differences between salt stress and alkali stress in cotton provides a theoretical basis for cotton cultivation on different types of saline alkali land.In this experiment,three treatments of CK,NaCl(CS),and NaHCO3+Na2CO3(AS)were conducted.Proteomics analysis and physiological index verification revealed that the tolerance mechanism of cotton to salt stress and alkali stress.Com-pared with CK,CS,and AS cotton plants decreased by 51.1%and 50.9%at P＜0.05 in biomass,respectively.The chlorophyll content and Pn under salt stress decreased significantly by 53.9%and 57.2%,respectively.Under salt stress,the activities of hexokinase,fructose phosphate kinase,pyruvate kinase,citrate synthase,glutamate dehydrogenase,and glutamic oxaloacetic transaminase in cotton leaves increased significantly by 13.8%,14.4%,4.7%,4.5%,36.6%,and 12.9%,respectively.Under alkali stress,the activities of hexokinase,phosphofructose kinase,pyruvate kinase,malate dehydrogenase,citrate synthase,glutamate dehydrogenase,and glutamic oxaloacetic transaminase in cotton leaves significantly increased by 4.8%,38.8%,15.1%,4.3%,3.4%,15.2%,and 21.1%,respectively.Based on TMT proteomics,458 differentially expressed proteins and 140 differentially expressed proteins in salt stress and alkali stress species were detected,respectively.These proteins were involved in photosynthe-sis,sugar metabolism,2-oxocarbonic acid metabolism,amino acid synthesis and metabolism,and other life processes.These re-sults showed that both salt stress and alkali stress inhibited cotton growth.The difference was that under salt stress,proteins ex-pression related to photosynthesis decreased,photosynthesis was significantly inhibited,and carbon water and energy metabolism were enhanced,and more photosynthates were used for energy metabolism.Alkali stress had no significant effect on cotton pho-tosynthesis,and more photosynthates might be transported to the roots to secrete organic acids.关键词
盐胁迫/碱胁迫/棉花/蛋白质组/光合作用Key words
salt stress/alkali stress/cotton/proteome/photosynthesis引用本文复制引用
郭家鑫,叶扬,郭慧娟,闵伟..盐碱胁迫对棉花叶片蛋白质组的影响及差异性分析[J].作物学报,2024,50(1):219-236,18.基金项目
本研究由石河子大学青年创新人才培养计划项目(CXPY202111),农业农村部西北绿洲农业环境重点实验室开放基金(XBLZ-20214)和石河子大学高层次人才科研启动资金专项(RCZK202017)资助. This study was supported by the Youth Innovation Talent Cultivation Program of Shihezi University(CXPY202111),the Open Fund of Key Laboratory of Northwest Oasis Agro-Environment of Ministry of Agriculture and Rural Affairs of China(XBLZ-20214),and the Scientific Research Starting Foundation for High Level Talents of Shihezi University(RCZK202017). （CXPY202111）
