盐碱胁迫对棉花叶片蛋白质组的影响及差异性分析OA
Effects and variability analysis of different salt and alkali stresses on the proteome of cotton leaves
盐胁迫和碱胁迫是严重影响农业生产的 2 种不同非生物胁迫,探讨棉花耐受盐胁迫和碱胁迫的差异,可为不同类型盐碱地的棉花栽培提供一定的理论基础.本试验设置对照(CK)、盐胁迫(NaCl,CS)和碱胁迫(NaHCO3+Na2CO3,AS)3 个处理,通过蛋白质组学分析和生理指标进行验证,揭示棉花对盐胁迫和碱胁迫的耐受机制.与CK相比,CS和AS的棉花生物量分别降低 51.1%和 50.9%,CS棉花叶片的叶绿素含量和净光合速率分别降低53.9%和 57.2%,CS棉花叶片中己糖激酶、磷酸果糖激酶、丙酮酸激酶、柠檬酸合酶、谷氨酸脱氢酶和谷草转氨酶活性分别增加 13.8%、14.4%、4.7%、4.5%、36.6%和 12.9%;AS棉花叶片中己糖激酶、磷酸果糖激酶、丙酮酸激酶、苹果酸脱氢酶、柠檬酸合酶、谷氨酸脱氢酶和谷草转氨酶活性分别增加 4.8%、38.8%、15.1%、4.3%、3.4%、15.2%和 21.1%.基于TMT蛋白质组学分析,在CS和AS叶片中分别鉴定出 458 个和 140 个差异表达蛋白质.这些蛋白参与了光合作用、糖代谢、2-氧羧酸代谢以及氨基酸合成和代谢等生命过程.表明盐胁迫和碱胁迫均抑制棉花生长.盐胁迫下,与光合作用相关的蛋白表达减少,光合作用被显著抑制,同时碳水和能量代谢加强,更多的光合产物用于能量代谢;而碱胁迫对棉花光合作用无显著影响,更多的光合产物可能运往根部分泌有机酸.
Salt stress and alkali stress are two different abiotic stresses that seriously affect agricultural production.Exploring the differences between salt stress and alkali stress in cotton provides a theoretical basis for cotton cultivation on different types of saline alkali land.In this experiment,three treatments of CK,NaCl(CS),and NaHCO3+Na2CO3(AS)were conducted.Proteomics analysis and physiological index verification revealed that the tolerance mechanism of cotton to salt stress and alkali stress.Com-pared with CK,CS,and AS cotton plants decreased by 51.1%and 50.9%at P＜0.05 in biomass,respectively.The chlorophyll content and Pn under salt stress decreased significantly by 53.9%and 57.2%,respectively.Under salt stress,the activities of hexokinase,fructose phosphate kinase,pyruvate kinase,citrate synthase,glutamate dehydrogenase,and glutamic oxaloacetic transaminase in cotton leaves increased significantly by 13.8%,14.4%,4.7%,4.5%,36.6%,and 12.9%,respectively.Under alkali stress,the activities of hexokinase,phosphofructose kinase,pyruvate kinase,malate dehydrogenase,citrate synthase,glutamate dehydrogenase,and glutamic oxaloacetic transaminase in cotton leaves significantly increased by 4.8%,38.8%,15.1%,4.3%,3.4%,15.2%,and 21.1%,respectively.Based on TMT proteomics,458 differentially expressed proteins and 140 differentially expressed proteins in salt stress and alkali stress species were detected,respectively.These proteins were involved in photosynthe-sis,sugar metabolism,2-oxocarbonic acid metabolism,amino acid synthesis and metabolism,and other life processes.These re-sults showed that both salt stress and alkali stress inhibited cotton growth.The difference was that under salt stress,proteins ex-pression related to photosynthesis decreased,photosynthesis was significantly inhibited,and carbon water and energy metabolism were enhanced,and more photosynthates were used for energy metabolism.Alkali stress had no significant effect on cotton pho-tosynthesis,and more photosynthates might be transported to the roots to secrete organic acids.
郭家鑫;叶扬;郭慧娟;闵伟
石河子大学农学院,新疆石河子 832000
盐胁迫碱胁迫棉花蛋白质组光合作用
salt stressalkali stresscottonproteomephotosynthesis
《作物学报》 2024 (001)
219-236 / 18
本研究由石河子大学青年创新人才培养计划项目(CXPY202111),农业农村部西北绿洲农业环境重点实验室开放基金(XBLZ-20214)和石河子大学高层次人才科研启动资金专项(RCZK202017)资助. This study was supported by the Youth Innovation Talent Cultivation Program of Shihezi University(CXPY202111),the Open Fund of Key Laboratory of Northwest Oasis Agro-Environment of Ministry of Agriculture and Rural Affairs of China(XBLZ-20214),and the Scientific Research Starting Foundation for High Level Talents of Shihezi University(RCZK202017).
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