作物学报2024，Vol.50Issue(1)：237-250,14.DOI:10.3724/SP.J.1006.2024.34076
甘蓝型油菜盐胁迫响应基因表达谱分析及共表达网络的构建
Relative expression profiles of genes response to salt stress and constructions of gene co-expression networks in Brassica napus L.
摘要
Abstract
Brassica napus L.is an important oil crop.Salt stress is one of the major environmental conditions affecting the growth and development of B.napus,which may lead to yield reduction,quality deterioration,and even the death of B.napus.In this study,the B.napus semi-winter cultivar ZS11 was used as the experimental material to perform transcriptome sequencing on the leaf and root tissues with salt stress treatment(0,0.25,0.5,1,3,6,12,and 24 h).The measured 90 RNA-seq data provided the high-resolution time dynamic transcriptional expression spectrum of rapeseed tissues responding to salt stress was obtained.Cor-relation analysis showed that the samples exhibited significant clustering differences in early response and late response before and after 1 h salt stress treatment.Using DESeq2 for differential gene analysis,we identified 20,462 and 29,334 differential genes for the response of root and leaf tissues,respectively,indicating that the overall response of leaf tissue in rapeseed was more se-vere than the root.Furthermore,WGCNA was used to construct gene co-expression networks for the salt stress response in root and leaf tissues,respectively,and tan and yellow modules were significantly related to the early response to salt stress,and green and red modules were significantly related to the late response to salt stress.GO enrichment analysis was then performed,and 41 and 26 core transcription factors responding to salt stress at the early and late stages,respectively,were selected from these net-works.Functional annotation showed that the known Arabidopsis homologous genes involved in salt stress responses at different stages existed in all four modules,and core genes,such as BnWRKY46 and BnWRKY57,had abundant SNPs variation and haplo-types in 505 salt stress-treated rape population variation data,suggesting that these core transcription factors might be key candi-date genes for rape salt stress response.This study provides a reliable data reference and candidate gene resources for improving salt tolerance in B.napus.关键词
甘蓝型油菜/RNA-seq/盐胁迫/WGCNA/基因共表达网络Key words
Brassica napus L./RNA-seq/salt stress/WGCNA/gene co-expression networks引用本文复制引用
杨闯,王玲,全成滔,余良倩,戴成,郭亮,傅廷栋,马朝芝..甘蓝型油菜盐胁迫响应基因表达谱分析及共表达网络的构建[J].作物学报,2024,50(1):237-250,14.基金项目
本研究由国家重点研发计划项目(2021YFD160014101-02)和湖北省重点研发计划项目(2020BBB061)资助. This study was supported by the National Key Research and Development Program of China(2021YFD160014101-02)and the National Key Research and Development Program of Hubei Province(2020BBB061). （2021YFD160014101-02）
