中医药学报2023，Vol.51Issue(12)：15-21,7.DOI:10.19664/j.cnki.1002-2392.230250
当归散对克罗米芬诱导的大鼠低容受性子宫内膜的作用机制研究
Study on the Mechanism of Danggui Powder on Clomiphene-Induced Hyporeceptive Endometrium in Rats
摘要
Abstract
Objective:To study the intervention effect of Danggui powder on clomiphene-induced hyporeceptive endometrium,and explore the difference of influence and pharmacodynamic mechanism of Danggui powder and its disassembled prescriptions.Methods:60 rats were randomly divided into normal group,model group,Huangqin(scutellaria baicalensis)and Baizhu(atractylodes macrocephala)group(HB group),Danggui powder group(DP group),Chuanxiong(Ligusticum wallichii),Danggui(paeonia lactiflora)and Shaoyao(Angelica sinensis)Group(CDS group),and estradiol valerate group(EV group)according to the body weight,with 10 rats in each group.On the first day after estrus,the model group and each treatment group were given clomiphene suspension(5.21 mg/kg)daily for 5 consecutive days.Meanwhile,the treatment groups were given estradiol valerate(0.104 mg/kg),Danggui powder,CDD,HB solutions(0.3125 g/kg,0.208 g/kg,0.104 g/kg)respectively for 8 consecutive days.Estrogen and progestogen were measured by radioimmunoassay;endometrial thickness and the number of glands of the rats were measured by HE staining;the expression of estrogen and progestogen receptors,and integrin β3 were determined by immunohistochemistry.Results:Compared with the normal group,the endometrial thickness,glands number,estrogen,estrogen receptor,estrogen/progestogen ratio and integrin β3 in the model group were significantly decreased(P<0.01),progestogen receptor expression decreased(P<0.05),and progestogen level increased(P<0.05).Compared with the model group,the endometrial thickness was significantly increased in each treatment group(P<0.05),among which DP group and CDS group were more significant(P<0.01);glands number was increased in each treatment group(P<0.05);estrogen level was increased in estradiol valerate group and CDS group(P<0.05);progestogen level was decreased in HB group(P<0.05),and the difference for other treatment groups were more significant(P<0.01);except for the HB group,estrogen/progestogen ratio in the other treatment groups was increased(P<0.05),and the difference in the CDS group was more significant(P<0.01);estrogen receptor was increased in every treatment group(P<0.05),among which estradiol valerate group and CDS group were more significant(P<0.01);except for HB group,integrin β3 of all the other treatment groups significantly increased(P<0.01).Conclusions:Danggui powder and its disassembled formulae can improve the endometrial morphologic development disorder induced by clomiphene in rats to varying degrees and enhance endometrial receptivity,which is related to estrogen,progestogen and its receptors,and integrin β3.关键词
当归散/雌激素/孕激素/整合素β3/子宫内膜容受性Key words
Danggui powder/Estrogen/Progestogen/Integrin β3/Endometrial receptivity分类
医药卫生引用本文复制引用
张楚欣,程宇立,胡红林,张若诗,高琳..当归散对克罗米芬诱导的大鼠低容受性子宫内膜的作用机制研究[J].中医药学报,2023,51(12):15-21,7.基金项目
国家自然科学基金项目(30801463) （30801463）
