中医药学报2023，Vol.51Issue(12)：22-28,7.DOI:10.19664/j.cnki.1002-2392.230251
芪苈强心胶囊抑制阿霉素诱导的心肌成纤维细胞活化的作用机制研究
Mechanism of Qili Cardiotonic Capsule Inhibiting Doxorubicin-Induced Activation of Myocardial Fibroblasts
摘要
Abstract
Objective:To investigate the effect of Qili cardiotonic capsule on doxorubicin-induced activation of cardiac fibroblasts(CFs)and its relationship with PAI-1/TGFβ1/Smad3 pathway.Methods:The cells were divided into control group,adriamycin model group,Qili cardiotonic low-dose group and Qili cardiotonic high-dose group.Real time fluorescence quantitative polymerase chain reaction(qPCR)and Western blot methods were used to detect type Ⅰ collagen(collagen Ⅰ),type Ⅲ collagen(collagen Ⅲ),plasminogen activator inhibitor-1(PAI-1),and transforming growth factor β1(TGFβ1),as well as mRNA and protein expression levels of Sma and Mad related protein 3(Smad3)in the cells of the four groups.Meanwhile,PAI-1 overexpression plasmid(PAI-1 OE)was transfected and divided into 4 groups:negative control group,adriamycin group,adriamycin + Qili cardiotonic group and adriamycin + Qili cardiotonic +PAI-1 OE group.The contents of collagen Ⅰ,collagen Ⅲ,TGFβ1 and p-Smad3 in cells of the four groups were detected by qPCR and western blot.Results:The relative protein expressions of collagen Ⅰ,collagen Ⅲ,PAI-1,TGFβ1 and P-SMad3 in adriamycin model group were significantly increased,while those in Qili cardiotonic low and high dose groups were down-regulated,with statistical significance(P<0.01).In the PAI-1 overexpression experiment,compared with adriamycin +Qili cardiotonic group,the protein contents of collagen Ⅰ,collagenⅢ,TGFβ1 and p-SMad3 in adriamycin + Qili cardiotonic + PAI-1 OE group were significantly increased,with statistical significance(P<0.01).These results suggested that overexpression of PAI-1 could significantly reverse the inhibitory effect of Qili cardiotonic capsule on TGFβ1 and p-Smad3.Conclusion:Qili cardiotonic capsule inhibits doxorubicin-induced CFs activation by inhibiting PAI-1/TGFβ1/Smad3 pathway.关键词
芪苈强心胶囊/心肌纤维化/PAI-1/TGFβ1/Smad3Key words
Qili cardiotonic capsule/Myocardial fibrosis/PAI-1/TGFβ1/Smad3分类
医药卫生引用本文复制引用
孙许涛,钟晴,张蓉,王博,霍金海,宋运佳,王伟明..芪苈强心胶囊抑制阿霉素诱导的心肌成纤维细胞活化的作用机制研究[J].中医药学报,2023,51(12):22-28,7.基金项目
国家自然科学基金青年基金项目(82205056) （82205056）
中国博士后资助项目(2022M711089) （2022M711089）
黑龙江省博士后基金(LBH-Z19035) （LBH-Z19035）
黑龙江中医药大学博士创新基金(2019BS06) （2019BS06）
