中医药学报2023，Vol.51Issue(12)：29-33,5.DOI:10.19664/j.cnki.1002-2392.230252
益脑通络颗粒对pMCAO模型大鼠SOD、MDA及NO水平的影响
Effects of Yinao Tongluo Granule on Levels of SOD,MDA and NO in pMCAO Model Rats
摘要
Abstract
Objective:To observe the effects of Yinao Tongluo Granule on serum levels of SOD,MDA and NO in pMCAO model rats.Methods:The rats were randomly divided into the sham operation group,the model group,the Yinao Tongluo Granule groups(low-dose,medium-dose and high-dose)and the ginkgo biloba group.The intervention was once per day for seven days.One week after intervention,suture-occluded method was applied to establish pMCAO model in rats;24 h after modeling,the Longa scoring method was adopted to evaluate nerve function score;water content was determined by dry/wet weight determination;TTC staining was used to determine the volume of cerebral infarction;TBA method was used to detect the plasma level of MDA;the serum level of SOD was detected by hydroxylamine method;the serum content of NO was detected by enzymic method.Results:Compared with those in the sham operation group,the neurological function score and the brain water content of the model group were significantly increased(P<0.05);compared with those in the model group,the neurological function score and the brain tissue water content were significantly decreased in the Yinao Tongluo Granule groups of medium-dose and high-dose,as well as in the Ginkgo biloba group(P<0.05).Compared with that in the sham operation group,the cerebral infarction volume was significantly increased in the model group(P<0.05);compared with that in the model group,the cerebral infarction volume was significantly decreased in the ginkgo biloba group and the Yinao Tongluo Granule groups of low-dose,medium-dose and high-dose(P<0.05);the cerebral infarction volume was less in the high-dose group than that in the ginkgo biloba group(P<0.05).Compared with those in the sham operation group,the SOD content was significantly decreased,the contents of MDA and NO were significantly increased in the model group(P<0.05);compared with those in the model group,the SOD content was significantly increased,and the contents of MDA and NO were significantly decreased in the ginkgo biloba group and the Yinao Tongluo Granule groups of medium-dose and high-dose(P<0.05);the SOD activity was higher and the levels of MDA and NO were lower in the high-dose group than those in the ginkgo biloba group(P<0.05).Conclusion:Yinao Tongluo Granule can reduce cerebral infarction volume and brain tissue water content in pMCAO model rats,it can increase SOD activity and decrease the levels of MDA and NO of pMCAO model rats,suggesting that Yinao Tongluo Granule can protect neurons and alleviate cerebral ischemia injury by inhibiting oxidative stress response.关键词
益脑通络颗粒/pMCAO模型/氧化应激反应/缺血性中风Key words
Yinao Tongluo Granule/pMCAO model/Oxidative stress response/Ischemic stroke分类
医药卫生引用本文复制引用
王振,张婷婷,宋莹,乌凯迪,李晓莹,陈阳燕,周明博,蒋希成..益脑通络颗粒对pMCAO模型大鼠SOD、MDA及NO水平的影响[J].中医药学报,2023,51(12):29-33,5.基金项目
国家自然科学基金项目(82174261) （82174261）
黑龙江省自然科学基金联合引导项目(LH2021H084) （LH2021H084）
黑龙江中医药大学科研基金新药基金项目(2019XY01) （2019XY01）
