安徽农业科学2023，Vol.51Issue(24)：1-4,4.DOI:10.3969/j.issn.0517-6611.2023.24.001
白星花金龟幼虫虫粪可培养微生物的分离鉴定及特性研究
Isolation,Identification and Characterization of Culturable Microorganisms from Frass of Protaetia brevitarsis Larva
摘要
Abstract
[Objective]To screen cultivable functional microorganisms,and provide microbial resources for the recycling of agricultural waste.[Method]Taking the frass of Protaetia brevitarsis larva with the edible fungi residues as the research object,the plate coating method was used to separate culturable microorganisms from feces,and their ability to decompose cellulose and protein was preliminarily explored.[Result]A to-tal of 5 strains of bacteria,and 3 strains of fungi were isolated.After identification,they were identified as Cellulosimicrobium cellulans,Entero-coccus casseliflavus,Arthrobacter protophormiae,Glutamicibacter halophytocola,Streptomyces griseolus,Candida tropicalis,Trichoderma longi-brachiatum,Geotrichum candidum.Among them,Cellulosimicrobium cellulans and Streptomyces griseolus had the ability to decompose protein,and the ratio of transparent circle diameter to colony diameter(HC)on casein culture medium were 7.259±0.417 and 1.971±0.584,re-spectively.Arthrobacter protophormiae,Streptomyces griseolus,Geotrichum candidum and Trichoderma longibrachiatum had the ability to degrade cellulose,and after Congo red staining on sodium carboxymethyl cellulose,the HC values of the transparent circle were 1.166±0.028,2.044±0.245,1.075±0.029 and 1.288±0.048.[Conclusion]Cellulosimicrobium cellulans,Arthrobacter protophormiae,Streptomyces griseolus,Geotrichum candidum and Trichoderma longibrachiatum can be used as strain resources for the reuse of agricultural wastes such as edible fungi residues.关键词
白星花金龟/幼虫虫粪/可培养微生物/分离鉴定Key words
Protaetia brevitarsis/Frass of larva/Culturable microorganisms/Isolation and identification分类
农业科学引用本文复制引用
张婷,王霄煜,吴如微,张莹莹,霍燃华,李海燕,任杰,李洁..白星花金龟幼虫虫粪可培养微生物的分离鉴定及特性研究[J].安徽农业科学,2023,51(24):1-4,4.基金项目
河北省重点研发计划项目(21327306D,20326807D). （21327306D,20326807D）
