安徽农业科学2023，Vol.51Issue(24)：127-130,4.DOI:10.3969/j.issn.0517-6611.2023.24.027
梨园土壤中梨火疫病菌存活时间研究
Study on Survival Time of Erwinia amylovora in Soil of Fragrant Pear Orchard
摘要
Abstract
In order to explore the existence of Erwinia amylovora in the soil of the diseased pear orchard and its survival time in the soil,from 2020 to 2021,soil was collected in the pear orchard of Korla City,Xinjiang,and the pathogenic bacteria were isolated by Zeller selective medi-um by streak separation method,and the pear fire blight bacterium E.amylovora was confirmed by combination of morphological identification,PCR detection and pathogenic test results,and E.amylovora in natural soil was continuously isolated.The results showed that there were pear fire blights in the soil of the diseased pear orchards.Pear fire blight bacteria could survive in soil for up to 550 days,and isolated strains re-mained significantly pathogenic.In this study,the existence and survival time of E.amylovora in the soil of pear orchards in Korla City were studied,and the results had important theoretical significance for the risk assessment of soil as the source of E.amylovora transmission,and pro-vided a scientific basis for the effective prevention and control of pear fire blight.关键词
土壤/Erwinia amylovora/存活时间/PCR/致病性/测试Key words
Soil/Erwinia amylovora/Survival time/PCR/Pathogenicity/Test分类
农业科技引用本文复制引用
李晓妹,韩丽丽,陈卫民..梨园土壤中梨火疫病菌存活时间研究[J].安徽农业科学,2023,51(24):127-130,4.基金项目
新疆科技厅重大科技专项(2019A01001). （2019A01001）
