安徽农业科学2023，Vol.51Issue(24)：173-178,6.DOI:10.3969/j.issn.0517-6611.2023.24.039
羌活根际土壤微生物群落结构特征及其对次生代谢产物的影响
Characteristics of Rhizosphere Soil Microbial Community Structure and Its Effects on Secondary Metabolites of Notopterygium inci-sum
摘要
Abstract
[Objective]To scientifically evaluate the health status of the soil ecosystem in the main production areas of N.incisum,and reveal the interaction relationship between soil microorganisms and N.incisum.[Method]Taking the rhizosphere soil of N.incisum as the research ob-ject,high-throughput sequencing technology and high-performance liquid chromatography(HPLC)were used to determine the composition of soil microbial community structure and the content of secondary metabolites of N.incisum.Furthermore,redundancy analysis and correlation a-nalysis methods were used to study the impact of rhizosphere soil microbiota on secondary metabolites of N.incisum.[Result]The results showed that the dominant bacterial at the phylum level were Proteobacteria,Acidobacteria and Bacteroidetes,and the Ascomycota,Basidiomyco-ta and Glomeromycota were identified as the dominant phyla of fungi.At the genus level,the dominant genera of soil bacteria were Vicinami-bacter,Terrimonas and nitrospira,which accounted for 0.80%-9.11%,0.66%-7.72%and 1.18%-4.462%,respectively.For fungi,the dominant genera were Mortierella,Tetracladium and Kotlabaca,which accounting for 0.01%-14.19%,0.18%-14.32%and 0.01%-24.96%,respectively.Redundancy analysis showed that the microbial community structure of the rhizosphere soil of N.incisum was mainly affected by soil pH and nutrient content.Colletotrichum and Botryichum showed significant negative correlation with pH(P＜0.05),while Bradyrhizobium also showed significant negative correlation with organic matter(P＜0.05).Candidatus_Udaeobate,Scutellospora and Enterocarpus were signif-icantly positively correlated with available phosphorus and available nitrogen(P＜0.05).The notopterol content were significantly negatively correlated with Chaetomium,Inocybe and Melanophyllum(P＜0.05),respectively,while isoimperatorin content was significantly positively correlated with Chaetomium and Humicola(P＜0.05),respectively.[Conclusion]The results of this study can provide data support for scientif-ic assessment of soil ecosystem characteristics and microbial-N.incisum interactions in the main N.incisum production area.关键词
羌活/根际土壤/微生物群落/结构特征/高通量测序/理化因子/次生代谢产物Key words
Notopterygium incisum/Rhizosphere soil/Microbial community/Structural characteristics/High-throughput sequencing/Physical and chemical factors/Secondary metabolites分类
农业科技引用本文复制引用
王红兰,朱文涛,崔俊芳,杨萍,杜玖珍,孙辉,周毅,蒋舜媛..羌活根际土壤微生物群落结构特征及其对次生代谢产物的影响[J].安徽农业科学,2023,51(24):173-178,6.基金项目
四川省省级公益性科研院所基本科研业务专项(2021JDKY-0013) （2021JDKY-0013）
四川省科技计划项目(2022NSFSC0102) （2022NSFSC0102）
四川省衔接推进乡村振兴科技专项(2022ZHXC0027) （2022ZHXC0027）
阿坝州科技计划项目(R21TJNLJS0001). （R21TJNLJS0001）
