安徽农业科学2023，Vol.51Issue(24)：183-186,190,5.DOI:10.3969/j.issn.0517-6611.2023.24.041
HPLC法同时测定不同产地新疆鼠李枝中3种黄酮类成分的含量
Simultaneous Determination of Three Flavonoids in the Branches of Rhamnus songorica from Different Regions of Xinjiang by HPLC
摘要
Abstract
[Objective]To establish an HPLC method for the simultaneous determination of quercetin,kaempferol and apigenin in Rhamnus songorica branches,and to optimize the extraction process of Rhamnus songorica branches and determine their content.[Method]The HPLC a-nalysis was carried out on Agilent XDB-C18 column(4.6 mm×250 mm,5 μm)with methanol(A)-0.4%phosphoric acid(B)gradient elu-tion(0-15 min,65%-55%B;15-35 min,55%-55%B;35-60 min,55%-40%B;60-70 min,40%-20%B;70-80 min,20%-65%B);ata-flow rate of 1 mL/min.The detection wave-length was 360 nm and the column temperature was 30℃.[Result]Good linear relationship were found in the the range of 6.89-110.20 μg/mL for quercetin,6.81-108.90 μg/mL for kaempferol,and 5.40-108.10 μg/mL for apigeni(r≥0.999 6).The average recovery rates of the above three components in the branches of Rhamnus songorica were 100.00%,99.01%and 100.43%,respectively,with RSDs of 1.26%,1.76%and 0.97%.[Conclusion]This method is accurate,reliable,and has good repeatability,providing an important basis for the quality evaluation and control standards of Rhamnus songorica branch extract.关键词
新疆鼠李/高效液相色谱法/槲皮素/山奈酚/芹菜素/含量测定Key words
Rhamnus songorica/HPLC/Quercetin/Kaempferol/Apigeni/Content determination分类
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叶斯木·塔拉甫别克,叶力夏提·达那别克,郭君婷,赵婷婷,刘桂花..HPLC法同时测定不同产地新疆鼠李枝中3种黄酮类成分的含量[J].安徽农业科学,2023,51(24):183-186,190,5.基金项目
新疆维吾尔自治区公益性科研院所基本业务费专项资金项目(KY2019105). （KY2019105）
