安徽中医药大学学报2023，Vol.42Issue(6)：39-44,6.DOI:10.3969/j.issn.2095-7246.2023.06.010
电针预处理对心肌缺血再灌注损伤小鼠下丘脑腹内侧核神经元活动的影响
Effect of Electroacupuncture Pretreatment on the Activity of Neurons in the Ventromedial Hypo-thalamic Nucleus in Mice with Myocardial Ischemia-Reperfusion Injury
摘要
Abstract
Objective To investigate the effect of electroacupuncture(EA)pretreatment at heart channel points on the activity of neurons in the ventromedial hypothalamic nucleus(VMH)of mice with myocardial ischemia-reperfusion injury(MIRI),as well as the central regulatory mechanism of EA pretreatment against MIRI.Methods A total of 36 C57BL/6 mice were ran-domly divided into sham-operation group,model group,and EA group,with 12 mice in each group.The mice in the EA group were given EA pretreatment at bilateral Shenmen and Tongli points for 30 minutes each time,once a day for 7 days in total,and a model of MIRI was established within 1 hour after the last time of EA pretreatment;the mice in the model group and the sham-operation group were not given EA.The Powerlab multi-channel physiological recorder and Chart software were used to record and analyze the change in the voltage of ST segment before modeling,at 30 minutes of ischemia,and at 120 minutes of reperfusion;HE staining was used to observe the histopathological changes of the myocardium;immunofluorescent staining was used to observe the expression of c-fos in the VMH of mice in each group and the co-expression of c-fos with glutamate(Glu)orγ-aminobutyric acid(GABA)in the VMH of the mice in the model group;the electrical activities of neurons in the VMH were collected,analyzed,and compared.Results Compared with the sham-operation group,the model group had a sig-nificant increase in the voltage of ST segment at 30 minutes after ligation and 120 minutes of reperfusion(P＜0.05);HE stai-ning showed that the mice in the model group had severe myocardial injury,a significant increase in the number of c-fos-positive cells in the VMH(P＜0.05),a significant increase in the discharge frequency of pyramidal neurons in the VMH(P＜0.05),a significant reduction in the discharge frequency of interneurons(P＜0.05),and enhanced spectral energy of local field poten-tial and a relatively intensive discharge frequency of neurons in the VMH.Compared with the model group,the EA group had a significant reduction in the voltage of ST segment at 30 minutes after ligation and 120 minutes of reperfusion(P＜0.05);HE staining showed relatively mild myocardial injury,a significant reduction in the number of c-fos-positive cells in the VMH(P＜0.05),and a significant reduction in the discharge frequency of pyramidal neurons in the VMH(P＜0.05),while there was no significant difference in the discharge frequency of neurons(P＞0.05),and there was a reduction in the spectral energy of local field potential and a relatively low discharge frequency of neurons in the VMH.As for the mice in the model group,the co-ex-pression level of c-fos and Glu was significantly higher than that of c-fos and GABA in the VMH(P＜0.05).Conclusion EA pretreatment at heart meridian points can reduce the discharge frequency of pyramidal neurons and the expression of c-fos in the VMH of mice and alleviate myocardial injury.Glutamatergic neurons in the VMH are involved in the central integration mecha-nism of EA against MIRI.关键词
电针预处理/心肌缺血再灌注/下丘脑腹内侧核/神经元活动Key words
Electroacupuncture pretreatment/Myocardial ischemia-reperfusion injury/Ventromedial hypothalamic nucleus/Neuronal activity分类
医药卫生引用本文复制引用
陈翩翩,蔡荣林,周翔,周杰,王帅亚,舒琪,王茜伊,张帆,胡玲,余情..电针预处理对心肌缺血再灌注损伤小鼠下丘脑腹内侧核神经元活动的影响[J].安徽中医药大学学报,2023,42(6):39-44,6.基金项目
国家自然科学基金项目(82074536,82104999) （82074536,82104999）
安徽省自然科学基金项目(2108085Y30,2108085QH364) （2108085Y30,2108085QH364）
安徽省高校科学研究项目(2022AH020043,2022AH030062) （2022AH020043,2022AH030062）
